Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5256851..5257476 Replicon chromosome
Accession NZ_CP092759
Organism Klebsiella pneumoniae strain bat16

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MKZ45_RS25990 Protein ID WP_002882817.1
Coordinates 5256851..5257234 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MKZ45_RS25995 Protein ID WP_004150355.1
Coordinates 5257234..5257476 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKZ45_RS25975 (MKZ45_25965) 5254217..5255119 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MKZ45_RS25980 (MKZ45_25970) 5255116..5255751 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MKZ45_RS25985 (MKZ45_25975) 5255748..5256677 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MKZ45_RS25990 (MKZ45_25980) 5256851..5257234 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MKZ45_RS25995 (MKZ45_25985) 5257234..5257476 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MKZ45_RS26000 (MKZ45_25990) 5257681..5258598 + 918 WP_002882812.1 alpha/beta hydrolase -
MKZ45_RS26005 (MKZ45_25995) 5258612..5259553 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MKZ45_RS26010 (MKZ45_26000) 5259598..5260035 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MKZ45_RS26015 (MKZ45_26005) 5260032..5260892 - 861 WP_002882807.1 virulence factor BrkB family protein -
MKZ45_RS26020 (MKZ45_26010) 5260886..5261485 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T237104 WP_002882817.1 NZ_CP092759:c5257234-5256851 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T237104 NZ_LR778150:c4330278-4330105 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT237104 WP_004150355.1 NZ_CP092759:c5257476-5257234 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT237104 NZ_LR778150:c4330602-4330306 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References