Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4059010..4059629 Replicon chromosome
Accession NZ_CP092759
Organism Klebsiella pneumoniae strain bat16

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag MKZ45_RS20240 Protein ID WP_002892050.1
Coordinates 4059411..4059629 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag MKZ45_RS20235 Protein ID WP_002892066.1
Coordinates 4059010..4059384 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKZ45_RS20225 (MKZ45_20220) 4054162..4055355 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MKZ45_RS20230 (MKZ45_20225) 4055378..4058524 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
MKZ45_RS20235 (MKZ45_20230) 4059010..4059384 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
MKZ45_RS20240 (MKZ45_20235) 4059411..4059629 + 219 WP_002892050.1 HHA domain-containing protein Toxin
MKZ45_RS20245 (MKZ45_20240) 4059788..4060354 + 567 WP_002892030.1 maltose O-acetyltransferase -
MKZ45_RS20250 (MKZ45_20245) 4060326..4060466 - 141 WP_004147370.1 hypothetical protein -
MKZ45_RS20255 (MKZ45_20250) 4060487..4060957 + 471 WP_002892026.1 YlaC family protein -
MKZ45_RS20260 (MKZ45_20255) 4060932..4062383 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
MKZ45_RS20265 (MKZ45_20260) 4062484..4063182 + 699 WP_002892021.1 GNAT family protein -
MKZ45_RS20270 (MKZ45_20265) 4063179..4063319 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
MKZ45_RS20275 (MKZ45_20270) 4063319..4063582 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T237100 WP_002892050.1 NZ_CP092759:4059411-4059629 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T237100 NZ_LR778150:2782845-2782952 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT237100 WP_002892066.1 NZ_CP092759:4059010-4059384 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT237100 NZ_LR778150:c2782795-2782732 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References