Detailed information of TA system TA023709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 362410..362908
• Replicon: chromosome II
• Accession: NC_012580
• Organism: Vibrio cholerae M66-2

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: VCM66_RS15085
• Protein ID: WP_000589156.1
• Coordinates: 362410..362676 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: VCM66_RS15090
• Protein ID: WP_000643598.1
• Coordinates: 362663..362908 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VCM66_RS18830	357712..357813	+	102	WP_001921603.1	hypothetical protein	-
VCM66_RS15045	357803..358189	+	387	WP_000703163.1	VOC family protein	-
VCM66_RS15050	358384..358635	+	252	WP_001890111.1	TIGR03643 family protein	-
VCM66_RS18835	358608..358757	+	150	WP_071908333.1	DUF3265 domain-containing protein	-
VCM66_RS15055	358809..359162	+	354	WP_000384365.1	hypothetical protein	-
VCM66_RS15060	359374..359616	+	243	WP_000107461.1	hypothetical protein	-
VCM66_RS15065	359868..360146	+	279	WP_000280324.1	DUF3709 domain-containing protein	-
VCM66_RS19555	360521..360664	+	144	Protein_344	DUF645 family protein	-
VCM66_RS19560	360718..360756	+	39	WP_082798268.1	hypothetical protein	-
VCM66_RS15075	360968..361435	+	468	WP_001289288.1	OsmC family protein	-
VCM66_RS15080	361563..362225	+	663	WP_000960654.1	hypothetical protein	-
VCM66_RS15085	362410..362676	+	267	WP_000589156.1	BrnT family toxin	Toxin
VCM66_RS15090	362663..362908	+	246	WP_000643598.1	hypothetical protein	Antitoxin
VCM66_RS15095	363004..363798	+	795	WP_001911581.1	hypothetical protein	-
VCM66_RS18865	363901..364029	+	129	WP_071908338.1	general secretion pathway protein GspI	-
VCM66_RS15100	364090..364272	+	183	WP_000923182.1	DUF645 family protein	-
VCM66_RS15105	364469..364894	+	426	WP_000403005.1	GNAT family N-acetyltransferase	-
VCM66_RS15110	365034..365624	+	591	WP_000021480.1	hypothetical protein	-
VCM66_RS15115	365778..366137	+	360	WP_001071529.1	VOC family protein	-
VCM66_RS15120	366284..366937	+	654	WP_000895163.1	hypothetical protein	-
VCM66_RS18870	366966..367067	+	102	WP_079993098.1	DUF3265 domain-containing protein	-
VCM66_RS18875	367083..367598	+	516	Protein_358	lipocalin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	305869..409778	103909
	-	inside	Integron	-	-	310949..409402	98453

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T23709 WP_000589156.1 NC_012580:362410-362676 [Vibrio cholerae M66-2]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T23709 NC_012580:362410-362676 [Vibrio cholerae M66-2]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
ATTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT23709 WP_000643598.1 NC_012580:362663-362908 [Vibrio cholerae M66-2]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT23709 NC_012580:362663-362908 [Vibrio cholerae M66-2]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTAGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGCTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2