Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 2585..3228 Replicon plasmid unnamed2
Accession NZ_CP092755
Organism Klebsiella pneumoniae strain bat17

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag MKZ44_RS27550 Protein ID WP_001044770.1
Coordinates 2812..3228 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag MKZ44_RS27545 Protein ID WP_001261282.1
Coordinates 2585..2815 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKZ44_RS27520 (MKZ44_27510) 255..587 - 333 WP_004152339.1 hypothetical protein -
MKZ44_RS27525 (MKZ44_27515) 594..992 - 399 WP_004171440.1 hypothetical protein -
MKZ44_RS27530 (MKZ44_27520) 1018..1346 - 329 Protein_3 hypothetical protein -
MKZ44_RS27535 (MKZ44_27525) 1374..1682 - 309 WP_004152336.1 hypothetical protein -
MKZ44_RS27540 (MKZ44_27535) 2169..2628 - 460 Protein_5 hypothetical protein -
MKZ44_RS27545 (MKZ44_27540) 2585..2815 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MKZ44_RS27550 (MKZ44_27545) 2812..3228 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
MKZ44_RS27555 (MKZ44_27550) 3302..3991 + 690 Protein_8 AAA family ATPase -
MKZ44_RS27560 (MKZ44_27555) 4046..4743 + 698 WP_223174630.1 IS1 family transposase -
MKZ44_RS27565 (MKZ44_27560) 4759..4869 + 111 Protein_10 mercuric transport protein periplasmic component -
MKZ44_RS27570 (MKZ44_27565) 4905..5327 + 423 WP_001340589.1 organomercurial transporter MerC -
MKZ44_RS27575 (MKZ44_27570) 5379..7073 + 1695 WP_000105636.1 mercury(II) reductase -
MKZ44_RS27580 (MKZ44_27575) 7091..7453 + 363 WP_001277456.1 mercury resistance co-regulator MerD -
MKZ44_RS27585 (MKZ44_27580) 7450..7686 + 237 WP_000993386.1 broad-spectrum mercury transporter MerE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1A / blaOXA-9 / blaKPC-3 - 1..118840 118840


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T237086 WP_001044770.1 NZ_CP092755:2812-3228 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T237086 NZ_LR778149:4103312-4103647 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT237086 WP_001261282.1 NZ_CP092755:2585-2815 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT237086 NZ_LR778149:4103064-4103312 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References