Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5256625..5257250 Replicon chromosome
Accession NZ_CP092753
Organism Klebsiella pneumoniae strain bat17

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MKZ44_RS25975 Protein ID WP_002882817.1
Coordinates 5256625..5257008 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MKZ44_RS25980 Protein ID WP_004150355.1
Coordinates 5257008..5257250 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKZ44_RS25960 (MKZ44_25950) 5253991..5254893 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MKZ44_RS25965 (MKZ44_25955) 5254890..5255525 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MKZ44_RS25970 (MKZ44_25960) 5255522..5256451 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MKZ44_RS25975 (MKZ44_25965) 5256625..5257008 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MKZ44_RS25980 (MKZ44_25970) 5257008..5257250 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MKZ44_RS25985 (MKZ44_25975) 5257455..5258372 + 918 WP_002882812.1 alpha/beta hydrolase -
MKZ44_RS25990 (MKZ44_25980) 5258386..5259327 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MKZ44_RS25995 (MKZ44_25985) 5259372..5259809 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MKZ44_RS26000 (MKZ44_25990) 5259806..5260666 - 861 WP_002882807.1 virulence factor BrkB family protein -
MKZ44_RS26005 (MKZ44_25995) 5260660..5261259 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T237084 WP_002882817.1 NZ_CP092753:c5257008-5256625 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T237084 NZ_LR778149:c3694691-3694227 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT237084 WP_004150355.1 NZ_CP092753:c5257250-5257008 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT237084 NZ_LR778149:c3695026-3694691 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References