Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4671473..4672283 Replicon chromosome
Accession NZ_CP092753
Organism Klebsiella pneumoniae strain bat17

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag MKZ44_RS23170 Protein ID WP_002887280.1
Coordinates 4671473..4672006 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag MKZ44_RS23175 Protein ID WP_002887278.1
Coordinates 4672017..4672283 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKZ44_RS23165 (MKZ44_23160) 4670304..4671425 + 1122 WP_002887282.1 cupin domain-containing protein -
MKZ44_RS23170 (MKZ44_23165) 4671473..4672006 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
MKZ44_RS23175 (MKZ44_23170) 4672017..4672283 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
MKZ44_RS23180 (MKZ44_23175) 4672386..4673819 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
MKZ44_RS23185 (MKZ44_23180) 4673809..4674492 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
MKZ44_RS23190 (MKZ44_23185) 4674664..4676049 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
MKZ44_RS23195 (MKZ44_23190) 4676067..4676411 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T237081 WP_002887280.1 NZ_CP092753:c4672006-4671473 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T237081 NZ_LR778149:c2615203-2615030 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT237081 WP_002887278.1 NZ_CP092753:c4672283-4672017 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT237081 NZ_LR778149:c2615527-2615231 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References