Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4058783..4059402 Replicon chromosome
Accession NZ_CP092753
Organism Klebsiella pneumoniae strain bat17

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag MKZ44_RS20225 Protein ID WP_002892050.1
Coordinates 4059184..4059402 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag MKZ44_RS20220 Protein ID WP_002892066.1
Coordinates 4058783..4059157 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKZ44_RS20210 (MKZ44_20205) 4053935..4055128 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MKZ44_RS20215 (MKZ44_20210) 4055151..4058297 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
MKZ44_RS20220 (MKZ44_20215) 4058783..4059157 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
MKZ44_RS20225 (MKZ44_20220) 4059184..4059402 + 219 WP_002892050.1 HHA domain-containing protein Toxin
MKZ44_RS20230 (MKZ44_20225) 4059561..4060127 + 567 WP_002892030.1 maltose O-acetyltransferase -
MKZ44_RS20235 (MKZ44_20230) 4060099..4060239 - 141 WP_004147370.1 hypothetical protein -
MKZ44_RS20240 (MKZ44_20235) 4060260..4060730 + 471 WP_002892026.1 YlaC family protein -
MKZ44_RS20245 (MKZ44_20240) 4060705..4062156 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
MKZ44_RS20250 (MKZ44_20245) 4062257..4062955 + 699 WP_002892021.1 GNAT family protein -
MKZ44_RS20255 (MKZ44_20250) 4062952..4063092 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
MKZ44_RS20260 (MKZ44_20255) 4063092..4063355 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T237080 WP_002892050.1 NZ_CP092753:4059184-4059402 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T237080 NZ_CP092753:4059184-4059402 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT237080 WP_002892066.1 NZ_CP092753:4058783-4059157 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT237080 NZ_CP092753:4058783-4059157 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References