Detailed information of TA system TA237066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1234675..1235592
• Replicon: chromosome
• Accession: NZ_CP092750
• Organism: Bacillus velezensis strain KS04AU

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: MKF36_RS06650
• Protein ID: WP_007407256.1
• Coordinates: 1234846..1235592 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: MKF36_RS06645
• Protein ID: WP_003154807.1
• Coordinates: 1234675..1234845 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKF36_RS06605 (MKF36_06605)	1229909..1231531	+	1623	WP_042635123.1	pyocin knob domain-containing protein	-
MKF36_RS06610 (MKF36_06610)	1231544..1231915	+	372	WP_007407260.1	XkdW family protein	-
MKF36_RS06615 (MKF36_06615)	1231920..1232117	+	198	WP_007610833.1	XkdX family protein	-
MKF36_RS06620 (MKF36_06620)	1232174..1232935	+	762	WP_007407258.1	hypothetical protein	-
MKF36_RS06625 (MKF36_06625)	1232987..1233250	+	264	WP_003154815.1	hemolysin XhlA family protein	-
MKF36_RS06630 (MKF36_06630)	1233264..1233527	+	264	WP_003154813.1	phage holin	-
MKF36_RS06635 (MKF36_06635)	1233541..1234419	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
MKF36_RS06645 (MKF36_06645)	1234675..1234845	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
MKF36_RS06650 (MKF36_06650)	1234846..1235592	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
MKF36_RS06655 (MKF36_06655)	1235697..1236695	-	999	WP_020955696.1	inorganic phosphate transporter	-
MKF36_RS06660 (MKF36_06660)	1236708..1237325	-	618	WP_003154804.1	DUF47 domain-containing protein	-
MKF36_RS06665 (MKF36_06665)	1237611..1238927	-	1317	WP_007407254.1	amino acid permease	-
MKF36_RS06670 (MKF36_06670)	1239250..1240200	+	951	WP_007407253.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1201524..1249945	48421

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T237066 WP_007407256.1 NZ_CP092750:c1235592-1234846 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T237066 NZ_LR778148:c3647239-3646952 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT237066 WP_003154807.1 NZ_CP092750:c1234845-1234675 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT237066 NZ_LR778148:c3647478-3647239 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14