Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazE-MazF
Location 63925..64490 Replicon plasmid pLiSL39
Accession NZ_CP092744
Organism Leptospira interrogans strain FMAS_PD1

Toxin (Protein)


Gene name mazF Uniprot ID A0A0F6H5X2
Locus tag FH585_RS20965 Protein ID WP_000648189.1
Coordinates 63925..64173 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A0C5XAD8
Locus tag FH585_RS20970 Protein ID WP_000237066.1
Coordinates 64167..64490 (+) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH585_RS20950 (FH585_20955) 60315..60791 + 477 WP_000691823.1 hypothetical protein -
FH585_RS20955 (FH585_20960) 61025..61963 - 939 WP_000852605.1 Rpn family recombination-promoting nuclease/putative transposase -
FH585_RS20960 (FH585_20965) 62235..62684 - 450 WP_240739037.1 leucine-rich repeat domain-containing protein -
FH585_RS20965 (FH585_20970) 63925..64173 + 249 WP_000648189.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Toxin
FH585_RS20970 (FH585_20975) 64167..64490 + 324 WP_000237066.1 type II toxin-antitoxin system PemK/MazF family toxin Antitoxin
FH585_RS20975 (FH585_20980) 64714..64926 + 213 WP_000447737.1 hypothetical protein -
FH585_RS20980 (FH585_20985) 65005..65397 + 393 WP_225741611.1 hypothetical protein -
FH585_RS20985 (FH585_20990) 65743..65892 + 150 Protein_70 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
FH585_RS20995 (FH585_21000) 67211..67552 + 342 WP_137108924.1 endoribonuclease MazF -
FH585_RS21000 (FH585_21005) 67896..69134 + 1239 Protein_73 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..73333 73333


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

SpoVT-AbrB domain

(6-49)

Antitoxin

mRNA interferase PemK-like

(2-105)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9507.04 Da        Isoelectric Point: 5.1749

>T237063 WP_000648189.1 NZ_CP092744:63925-64173 [Leptospira interrogans]
MKASVVKIGNSKGIRIPKAVLEECHIEEEVDLLIDNNKLIIVPLKSKPREGWEKQFKTMSSNKDDKLFIPDSIDLLDKDW
EW

Download         Length: 249 bp

>T237063 NZ_LR778148:c3258690-3258583 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12168.59 Da        Isoelectric Point: 10.5161

>AT237063 WP_000237066.1 NZ_CP092744:64167-64490 [Leptospira interrogans]
MVISQYEVYLINLDPTVGHEIKKSRPCAIISPNEMNKTIGTIIIAPMTTKSRSYPTRVELTFQGKKGWIVLDQIRAVDKT
RLVKKLGKIDPKTVNKMKLVIKEMLVD

Download         Length: 324 bp

>AT237063 NZ_LR778148:3258737-3258803 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6H5X2

Antitoxin

Source ID Structure
AlphaFold DB A0A0C5XAD8

References