Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 1422148..1422724 Replicon chromosome
Accession NZ_CP092743
Organism Leptospira interrogans strain FMAS_PD1

Toxin (Protein)


Gene name chpK Uniprot ID Q93MT8
Locus tag FH585_RS07605 Protein ID WP_000617906.1
Coordinates 1422383..1422724 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID Q93MT9
Locus tag FH585_RS07600 Protein ID WP_000844758.1
Coordinates 1422148..1422396 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH585_RS07585 (FH585_07585) 1417808..1419790 + 1983 WP_000802798.1 GAF domain-containing protein -
FH585_RS07590 (FH585_07590) 1420424..1420882 - 459 WP_000453264.1 hypothetical protein -
FH585_RS07595 (FH585_07595) 1421036..1421332 + 297 WP_000477061.1 helix-turn-helix transcriptional regulator -
FH585_RS07600 (FH585_07600) 1422148..1422396 + 249 WP_000844758.1 ribbon-helix-helix domain-containing protein Antitoxin
FH585_RS07605 (FH585_07605) 1422383..1422724 + 342 WP_000617906.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FH585_RS07610 (FH585_07610) 1423749..1424741 - 993 WP_000041092.1 aldolase/citrate lyase family protein -
FH585_RS07615 (FH585_07615) 1424881..1425816 - 936 WP_000004530.1 ornithine carbamoyltransferase -
FH585_RS07620 (FH585_07620) 1426620..1427087 + 468 WP_000060052.1 hypothetical protein -
FH585_RS07625 (FH585_07625) 1427084..1427488 + 405 WP_001974506.1 pyridoxamine 5'-phosphate oxidase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12372.25 Da        Isoelectric Point: 6.3272

>T237060 WP_000617906.1 NZ_CP092743:1422383-1422724 [Leptospira interrogans]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGEVEIGLKLVTGLD

Download         Length: 342 bp

>T237060 NZ_LR778148:c2403455-2403237 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9380.77 Da        Isoelectric Point: 10.3815

>AT237060 WP_000844758.1 NZ_CP092743:1422148-1422396 [Leptospira interrogans]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT237060 NZ_LR778148:c2403855-2403481 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MS74

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HD92

References