Detailed information of TA system TA237059

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 1003120..1003748
• Replicon: chromosome
• Accession: NZ_CP092743
• Organism: Leptospira interrogans strain FMAS_PD1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q8F7E4
• Locus tag: FH585_RS06020
• Protein ID: WP_000281042.1
• Coordinates: 1003120..1003518 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q8F7E3
• Locus tag: FH585_RS06025
• Protein ID: WP_001192482.1
• Coordinates: 1003518..1003748 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH585_RS05985 (FH585_05985)	998636..999478	+	843	WP_137170910.1	hypothetical protein	-
FH585_RS05990 (FH585_05990)	999734..1000811	-	1078	Protein_818	hypothetical protein	-
FH585_RS05995 (FH585_05995)	1000922..1001182	-	261	WP_137108817.1	hypothetical protein	-
FH585_RS06000 (FH585_06000)	1001204..1001503	-	300	Protein_820	hypothetical protein	-
FH585_RS06005 (FH585_06005)	1001602..1001874	-	273	WP_240738869.1	BrnA antitoxin family protein	-
FH585_RS06010 (FH585_06010)	1001861..1002154	-	294	WP_061243121.1	BrnT family toxin	-
FH585_RS06015 (FH585_06015)	1002334..1002864	-	531	WP_061261813.1	hypothetical protein	-
FH585_RS06020 (FH585_06020)	1003120..1003518	-	399	WP_000281042.1	type II toxin-antitoxin system VapC family toxin	Toxin
FH585_RS06025 (FH585_06025)	1003518..1003748	-	231	WP_001192482.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FH585_RS06030 (FH585_06030)	1004116..1004460	-	345	WP_000392070.1	hypothetical protein	-
FH585_RS06035 (FH585_06035)	1004683..1005360	-	678	WP_000071998.1	hypothetical protein	-
FH585_RS06040 (FH585_06040)	1005592..1006281	-	690	WP_000590462.1	SH3 domain-containing protein	-
FH585_RS06045 (FH585_06045)	1006507..1007430	-	924	WP_000680711.1	M28 family peptidase	-
FH585_RS06050 (FH585_06050)	1007637..1008266	-	630	WP_001081629.1	S24 family peptidase	-
FH585_RS06055 (FH585_06055)	1008398..1008607	+	210	WP_000041838.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15139.76 Da
• Isoelectric Point: 9.6893

>T237059 WP_000281042.1 NZ_CP092743:c1003518-1003120 [Leptospira interrogans]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Nucleotide

• Length: 399 bp

>T237059 NZ_LR778148:c2189724-2189446 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8771.05 Da
• Isoelectric Point: 8.6588

>AT237059 WP_001192482.1 NZ_CP092743:c1003748-1003518 [Leptospira interrogans]
MQTAKLFINGRSQAVRLPKEFQFTGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEEREQPKSDKREKL

Nucleotide

• Length: 231 bp

>AT237059 NZ_LR778148:c2189987-2189727 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A0F6HAN6

Antitoxin

• AlphaFold DB: A0A0F6HAF7