Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 19510..20081 Replicon plasmid pLiSL37
Accession NZ_CP092740
Organism Leptospira interrogans strain FMAS_AW2

Toxin (Protein)


Gene name mazF Uniprot ID Q8F5A3
Locus tag FH582_RS21780 Protein ID WP_000240418.1
Coordinates 19510..19851 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M6U553
Locus tag FH582_RS21785 Protein ID WP_000443825.1
Coordinates 19845..20081 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH582_RS21740 (FH582_21730) 15336..15947 - 612 WP_000851074.1 hypothetical protein -
FH582_RS21745 (FH582_21735) 16055..16516 - 462 WP_000811790.1 hypothetical protein -
FH582_RS21750 (FH582_21740) 17039..17239 - 201 WP_017857623.1 hypothetical protein -
FH582_RS21755 (FH582_21745) 17554..17700 + 147 WP_167493406.1 hypothetical protein -
FH582_RS21760 (FH582_21750) 17663..18388 + 726 WP_000414903.1 hypothetical protein -
FH582_RS21765 (FH582_21755) 18391..18732 + 342 WP_000727287.1 hypothetical protein -
FH582_RS21770 (FH582_21760) 18793..19059 + 267 WP_000844450.1 hypothetical protein -
FH582_RS21775 (FH582_21765) 19059..19442 + 384 WP_000617796.1 hypothetical protein -
FH582_RS21780 (FH582_21770) 19510..19851 - 342 WP_000240418.1 endoribonuclease MazF Toxin
FH582_RS21785 (FH582_21775) 19845..20081 - 237 WP_000443825.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FH582_RS21790 (FH582_21780) 20421..21428 - 1008 WP_240737986.1 hypothetical protein -
FH582_RS21795 (FH582_21785) 21767..22003 - 237 WP_017860810.1 hypothetical protein -
FH582_RS21800 (FH582_21790) 22133..22480 + 348 WP_240299286.1 hypothetical protein -
FH582_RS21805 (FH582_21795) 22681..22881 - 201 WP_229266939.1 hypothetical protein -
FH582_RS21810 (FH582_21800) 22836..23270 - 435 WP_061226524.1 M15 family metallopeptidase -
FH582_RS21815 (FH582_21805) 23270..23731 - 462 WP_137119371.1 hypothetical protein -
FH582_RS21820 (FH582_21810) 23738..24181 - 444 WP_001129679.1 hypothetical protein -
FH582_RS21825 (FH582_21815) 24181..24561 - 381 WP_045193734.1 hypothetical protein -
FH582_RS21830 (FH582_21820) 24554..24916 - 363 WP_000821938.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..71951 71951


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-112)

Antitoxin

Antitoxin PemI-like

(1-78)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12918.12 Da        Isoelectric Point: 9.9595

>T237058 WP_000240418.1 NZ_CP092740:c19851-19510 [Leptospira interrogans]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Download         Length: 342 bp

>T237058 NZ_LR778148:1642302-1642475 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8728.95 Da        Isoelectric Point: 5.6663

>AT237058 WP_000443825.1 NZ_CP092740:c20081-19845 [Leptospira interrogans]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Download         Length: 237 bp

>AT237058 NZ_LR778148:1641978-1642274 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATCAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4D8SL30

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HC33

References