Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 4199930..4200558 Replicon chromosome
Accession NZ_CP092739
Organism Leptospira interrogans strain FMAS_AW2

Toxin (Protein)


Gene name vapC Uniprot ID Q8F7E4
Locus tag FH582_RS19855 Protein ID WP_000281042.1
Coordinates 4200160..4200558 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8F7E3
Locus tag FH582_RS19850 Protein ID WP_001192482.1
Coordinates 4199930..4200160 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH582_RS19825 (FH582_19815) 4195186..4195776 + 591 WP_000437520.1 energy transducer TonB -
FH582_RS19830 (FH582_19820) 4196102..4197646 - 1545 WP_017857865.1 YceK/YidQ family lipoprotein -
FH582_RS19835 (FH582_19825) 4197683..4198573 + 891 WP_000789618.1 hypothetical protein -
FH582_RS19840 (FH582_19830) 4198782..4199123 + 342 WP_000611885.1 hypothetical protein -
FH582_RS19845 (FH582_19835) 4199160..4199639 + 480 WP_002108895.1 hypothetical protein -
FH582_RS19850 (FH582_19840) 4199930..4200160 + 231 WP_001192482.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FH582_RS19855 (FH582_19845) 4200160..4200558 + 399 WP_000281042.1 type II toxin-antitoxin system VapC family toxin Toxin
FH582_RS19860 (FH582_19850) 4200656..4200955 + 300 Protein_3413 hypothetical protein -
FH582_RS19865 (FH582_19855) 4200977..4201204 + 228 WP_000130601.1 hypothetical protein -
FH582_RS19870 (FH582_19860) 4201347..4202423 + 1077 WP_001170569.1 hypothetical protein -
FH582_RS19875 (FH582_19865) 4202424..4202882 + 459 WP_001239004.1 GNAT family N-acetyltransferase -
FH582_RS19880 (FH582_19870) 4203098..4203958 - 861 WP_000682231.1 hypothetical protein -
FH582_RS19885 (FH582_19875) 4204499..4205266 - 768 WP_017857864.1 DUF1554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15139.76 Da        Isoelectric Point: 9.6893

>T237057 WP_000281042.1 NZ_CP092739:4200160-4200558 [Leptospira interrogans]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Download         Length: 399 bp

>T237057 NZ_LR778148:1363893-1364204 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8771.05 Da        Isoelectric Point: 8.6588

>AT237057 WP_001192482.1 NZ_CP092739:4199930-4200160 [Leptospira interrogans]
MQTAKLFINGRSQAVRLPKEFQFTGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEEREQPKSDKREKL

Download         Length: 231 bp

>AT237057 NZ_LR778148:1364205-1364495 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6HAN6

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HAF7

References