237055

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 2887836..2888412 Replicon chromosome
Accession NZ_CP092739
Organism Leptospira interrogans strain FMAS_AW2

Toxin (Protein)


Gene name chpK Uniprot ID Q93MT8
Locus tag FH582_RS14750 Protein ID WP_000617906.1
Coordinates 2887836..2888177 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID Q93MT9
Locus tag FH582_RS14755 Protein ID WP_000844758.1
Coordinates 2888164..2888412 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH582_RS14730 (FH582_14725) 2883071..2883475 - 405 WP_001974506.1 pyridoxamine 5'-phosphate oxidase family protein -
FH582_RS14735 (FH582_14730) 2883472..2883939 - 468 WP_000060051.1 hypothetical protein -
FH582_RS14740 (FH582_14735) 2884743..2885678 + 936 WP_000004530.1 ornithine carbamoyltransferase -
FH582_RS14745 (FH582_14740) 2885818..2886810 + 993 WP_000041092.1 aldolase/citrate lyase family protein -
FH582_RS14750 (FH582_14745) 2887836..2888177 - 342 WP_000617906.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FH582_RS14755 (FH582_14750) 2888164..2888412 - 249 WP_000844758.1 ribbon-helix-helix domain-containing protein Antitoxin
FH582_RS14760 (FH582_14755) 2889228..2889524 - 297 WP_000477061.1 helix-turn-helix transcriptional regulator -
FH582_RS14765 (FH582_14760) 2889678..2890136 + 459 WP_000453263.1 hypothetical protein -
FH582_RS14770 (FH582_14765) 2890778..2892760 - 1983 WP_000802799.1 HD family phosphohydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12372.25 Da        Isoelectric Point: 6.3272

>T237055 WP_000617906.1 NZ_CP092739:c2888177-2887836 [Leptospira interrogans]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGEVEIGLKLVTGLD

Download         Length: 342 bp

>T237055 NZ_LR778148:559677-560141 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9380.77 Da        Isoelectric Point: 10.3815

>AT237055 WP_000844758.1 NZ_CP092739:c2888412-2888164 [Leptospira interrogans]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT237055 NZ_LR778148:559342-559677 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MS74

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HD92

References