Detailed information of TA system TA237053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazE-MazF
• Location: 62615..63180
• Replicon: plasmid pLiSL36
• Accession: NZ_CP092738
• Organism: Leptospira interrogans strain FMAS_AW2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0F6H5X2
• Locus tag: FH582_RS02510
• Protein ID: WP_000648189.1
• Coordinates: 62615..62863 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A0C5XAD8
• Locus tag: FH582_RS02515
• Protein ID: WP_000237066.1
• Coordinates: 62857..63180 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH582_RS02480	57752..57973	+	222	WP_017858173.1	hypothetical protein	-
FH582_RS02485 (FH582_02480)	58232..58726	-	495	WP_017858174.1	hypothetical protein	-
FH582_RS02490 (FH582_02485)	58973..59476	+	504	WP_000614121.1	hypothetical protein	-
FH582_RS02495 (FH582_02490)	59608..60096	+	489	WP_002131542.1	DUF4942 domain-containing protein	-
FH582_RS02500 (FH582_02495)	60216..60461	+	246	WP_000695324.1	hypothetical protein	-
FH582_RS02505 (FH582_02500)	61346..61651	+	306	WP_000844596.1	hypothetical protein	-
FH582_RS02510 (FH582_02505)	62615..62863	+	249	WP_000648189.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Toxin
FH582_RS02515 (FH582_02510)	62857..63180	+	324	WP_000237066.1	type II toxin-antitoxin system PemK/MazF family toxin	Antitoxin
FH582_RS02520 (FH582_02515)	63288..63473	+	186	Protein_58	toxin-antitoxin system, toxin component	-
FH582_RS02525 (FH582_02520)	63537..63716	-	180	WP_000536132.1	hypothetical protein	-
FH582_RS02530 (FH582_02525)	63632..64393	+	762	Protein_60	hypothetical protein	-
FH582_RS02535 (FH582_02530)	64686..64877	+	192	Protein_61	AraC family transcriptional regulator	-
FH582_RS02540 (FH582_02535)	65262..66140	-	879	Protein_62	kelch repeat-containing protein	-
FH582_RS02545 (FH582_02540)	66390..67463	+	1074	WP_162303305.1	IS110-like element ISLin1 family transposase	-
FH582_RS22180	67502..67820	-	319	Protein_64	galactose oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..84077	84077
	-	flank	IS/Tn	-	-	66390..67463	1073

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• SpoVT-AbrB domain (6-49)

Antitoxin

• mRNA interferase PemK-like (2-105)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9507.04 Da
• Isoelectric Point: 5.1749

>T237053 WP_000648189.1 NZ_CP092738:62615-62863 [Leptospira interrogans]
MKASVVKIGNSKGIRIPKAVLEECHIEEEVDLLIDNNKLIIVPLKSKPREGWEKQFKTMSSNKDDKLFIPDSIDLLDKDW
EW

Nucleotide

• Length: 249 bp

>T237053 NZ_LR778148:c151058-150723 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12168.59 Da
• Isoelectric Point: 10.5161

>AT237053 WP_000237066.1 NZ_CP092738:62857-63180 [Leptospira interrogans]
MVISQYEVYLINLDPTVGHEIKKSRPCAIISPNEMNKTIGTIIIAPMTTKSRSYPTRVELTFQGKKGWIVLDQIRAVDKT
RLVKKLGKIDPKTVNKMKLVIKEMLVD

Nucleotide

• Length: 324 bp

>AT237053 NZ_LR778148:c151306-151058 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0F6H5X2

Antitoxin

• AlphaFold DB: A0A0C5XAD8