Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 109985..110556 Replicon plasmid pLiSL35
Accession NZ_CP092737
Organism Leptospira interrogans strain FMAS_AW2

Toxin (Protein)


Gene name mazF Uniprot ID Q8F5A3
Locus tag FH582_RS02070 Protein ID WP_000240418.1
Coordinates 110215..110556 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M6U553
Locus tag FH582_RS02065 Protein ID WP_000443825.1
Coordinates 109985..110221 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH582_RS02015 (FH582_02015) 105140..105520 + 381 WP_045193734.1 hypothetical protein -
FH582_RS02020 (FH582_02020) 105520..105963 + 444 WP_001129679.1 hypothetical protein -
FH582_RS02025 (FH582_02025) 105970..106431 + 462 WP_137119371.1 hypothetical protein -
FH582_RS02030 (FH582_02030) 106431..106865 + 435 WP_061226524.1 M15 family metallopeptidase -
FH582_RS02035 (FH582_02035) 106820..107020 + 201 WP_000340565.1 hypothetical protein -
FH582_RS02040 (FH582_02040) 107070..107303 + 234 WP_000416314.1 hypothetical protein -
FH582_RS02045 (FH582_02045) 107300..107740 + 441 WP_001289702.1 hypothetical protein -
FH582_RS02050 (FH582_02050) 107730..107945 + 216 WP_017857593.1 hypothetical protein -
FH582_RS02055 (FH582_02055) 108115..108303 + 189 WP_000425065.1 hypothetical protein -
FH582_RS02060 (FH582_02060) 108638..109645 + 1008 WP_240737986.1 hypothetical protein -
FH582_RS02065 (FH582_02065) 109985..110221 + 237 WP_000443825.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FH582_RS02070 (FH582_02070) 110215..110556 + 342 WP_000240418.1 endoribonuclease MazF Toxin
FH582_RS02075 (FH582_02075) 110624..111007 - 384 WP_000617796.1 hypothetical protein -
FH582_RS02080 (FH582_02080) 111007..111273 - 267 WP_000844450.1 hypothetical protein -
FH582_RS02085 (FH582_02085) 111334..111675 - 342 WP_000727287.1 hypothetical protein -
FH582_RS02090 (FH582_02090) 111678..112403 - 726 WP_000414903.1 hypothetical protein -
FH582_RS02095 (FH582_02095) 112366..112512 - 147 WP_167493406.1 hypothetical protein -
FH582_RS02100 (FH582_02100) 112827..113027 + 201 WP_017857623.1 hypothetical protein -
FH582_RS02105 (FH582_02105) 113550..114011 + 462 WP_000811790.1 hypothetical protein -
FH582_RS02110 (FH582_02110) 114119..114730 + 612 WP_000851074.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..142315 142315


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-112)

Antitoxin

Antitoxin PemI-like

(1-78)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12918.12 Da        Isoelectric Point: 9.9595

>T237052 WP_000240418.1 NZ_CP092737:110215-110556 [Leptospira interrogans]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Download         Length: 342 bp

>T237052 NZ_LR778147:c5043397-5043047 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8728.95 Da        Isoelectric Point: 5.6663

>AT237052 WP_000443825.1 NZ_CP092737:109985-110221 [Leptospira interrogans]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Download         Length: 237 bp

>AT237052 NZ_LR778147:c5043642-5043391 [Escherichia coli]
ATGCGTATTACCATAAAAAAATGGGGGAACAGTTCAGGGATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4D8SL30

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HC33

References