Detailed information of TA system TA237050

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 3715795..3717485
• Replicon: chromosome
• Accession: NZ_CP092733
• Organism: Comamonas thiooxydans strain CHJ01

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: MJ608_RS17390
• Protein ID: WP_039050302.1
• Coordinates: 3716607..3717485 (-)
• Length: 293 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A4Y8THJ5
• Locus tag: MJ608_RS17385
• Protein ID: WP_004340321.1
• Coordinates: 3715795..3716601 (-)
• Length: 269 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ608_RS17360 (MJ608_17330)	3710939..3711796	-	858	WP_012840239.1	IclR family transcriptional regulator	-
MJ608_RS17365 (MJ608_17335)	3711804..3712580	-	777	WP_039050300.1	SDR family oxidoreductase	-
MJ608_RS17370 (MJ608_17340)	3712895..3713653	-	759	WP_054074331.1	5'-methylthioadenosine/adenosylhomocysteine nucleosidase	-
MJ608_RS17375 (MJ608_17345)	3713773..3714759	-	987	WP_012840242.1	tripartite tricarboxylate transporter substrate binding protein	-
MJ608_RS17380 (MJ608_17350)	3714952..3715752	-	801	WP_256629102.1	aspartate dehydrogenase	-
MJ608_RS17385 (MJ608_17355)	3715795..3716601	-	807	WP_004340321.1	IclR family transcriptional regulator	Antitoxin
MJ608_RS17390 (MJ608_17360)	3716607..3717485	-	879	WP_039050302.1	alpha/beta hydrolase	Toxin
MJ608_RS17395 (MJ608_17365)	3717494..3718027	-	534	WP_003072476.1	cupin domain-containing protein	-
MJ608_RS17400 (MJ608_17370)	3718287..3719963	-	1677	WP_012840246.1	long-chain-fatty-acid--CoA ligase	-
MJ608_RS17405 (MJ608_17375)	3720177..3721241	-	1065	WP_004340312.1	fructose-bisphosphate aldolase class II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (44-277)

Antitoxin

• Transcription regulator IclR, C-terminal (137-257)

Sequences

Toxin

Protein

• Length: 293 a.a.
• Molecular weight: 30687.17 Da
• Isoelectric Point: 6.6011

>T237050 WP_039050302.1 NZ_CP092733:c3717485-3716607 [Comamonas thiooxydans]
MHEDLLSLKQQLLQTGFPERLVKLPQKAQVAWREAGDASSDFAVVLLHGISSGAASWLDVALQLGDKARVLAWDAPGYGV
STPLAQSAPADADYAQVLAQSLLVLGVRRCILVGHSLGALMAARLAVTAAPGLVEQLVLISPAGGYGAPAKAEQQAKVRE
GRLAALAEKGVAGLAAVIDQRLVSSEAPESVRAWVRWNTARMQPQGYAQAVELLCGSDLAQARGRLGMPVEVWVGEHDVV
TTPAACKSWSELLGAHYGTLAAAGHASPVEQPMEVAHRLASLLNQSYCPNTS

Nucleotide

• Length: 879 bp

>T237050 NZ_LR778147:c4782970-4782782 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCTCCAGAACGGCTTAACA
CAATCCTTTTACTGGTAGACGGCTCATGA

Antitoxin

Protein

• Length: 269 a.a.
• Molecular weight: 29259.22 Da
• Isoelectric Point: 5.3811

>AT237050 WP_004340321.1 NZ_CP092733:c3716601-3715795 [Comamonas thiooxydans]
MSETSEISTNEQDRYTVPALDRGLRLLACFGPAQPVWSAPELARSLELPRSTVFRMLTTLENSGYLQRSGTEYRLGLAVL
RLGYDYLSTQPLAQLAEPVLQALCDELGMTSNLALRDGTSVVYVARVTPSGAFQGAVRVGSRLPAHATVLGRALLHDMDG
AQLRSVFGGEELPQFSESTPRNIDELLRLLAEDRERGYAMGEGFYEPGVSSIAAPVRAADGRVVAGLAMAIPFTELGPEK
TQLWVQKVQQAAETLSAHLRQQHPDHLR

Nucleotide

• Length: 807 bp

>AT237050 NZ_LR778147:c4783206-4782973 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A4Y8THJ5