23705

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 348510..349276 Replicon chromosome II
Accession NC_012580
Organism Vibrio cholerae M66-2

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag VCM66_RS14965 Protein ID WP_000982260.1
Coordinates 348510..349007 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag VCM66_RS14970 Protein ID WP_000246253.1
Coordinates 349004..349276 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VCM66_RS14920 343952..344656 + 705 WP_000087610.1 HNH endonuclease -
VCM66_RS14925 344814..345152 + 339 WP_000713853.1 hypothetical protein -
VCM66_RS14930 345306..345623 - 318 WP_001180243.1 type II toxin-antitoxin system RelE/ParE family toxin -
VCM66_RS14935 345642..345884 - 243 WP_000557292.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
VCM66_RS14940 346128..346514 + 387 WP_001015867.1 NUDIX hydrolase -
VCM66_RS18770 346571..346684 + 114 WP_001900214.1 hypothetical protein -
VCM66_RS14945 346633..346914 + 282 WP_001894429.1 DUF1289 domain-containing protein -
VCM66_RS14955 347172..347708 + 537 WP_000469482.1 GNAT family N-acetyltransferase -
VCM66_RS14960 347845..348360 + 516 WP_001201509.1 lipocalin family protein -
VCM66_RS14965 348510..349007 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
VCM66_RS14970 349004..349276 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
VCM66_RS18790 349669..349794 + 126 WP_001900195.1 DUF645 family protein -
VCM66_RS19320 349810..349848 + 39 WP_106019118.1 hypothetical protein -
VCM66_RS14980 350044..350490 + 447 WP_000006157.1 hypothetical protein -
VCM66_RS14985 350638..350907 - 270 WP_000277238.1 type II toxin-antitoxin system YafQ family toxin -
VCM66_RS14990 350900..351178 - 279 WP_001258569.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
VCM66_RS14995 351428..351685 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
VCM66_RS15000 351673..351975 + 303 WP_000229318.1 type II toxin-antitoxin system RelE/ParE family toxin -
VCM66_RS18795 352264..352488 + 225 WP_001894423.1 DUF3709 domain-containing protein -
VCM66_RS18800 352932..353051 + 120 WP_071919600.1 DUF645 family protein -
VCM66_RS19335 353055..353108 + 54 Protein_329 DUF645 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 305869..409778 103909
- inside Integron - - 310949..409402 98453


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T23705 WP_000982260.1 NC_012580:c349007-348510 [Vibrio cholerae M66-2]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T23705 NC_012580:c349007-348510 [Vibrio cholerae M66-2]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT23705 WP_000246253.1 NC_012580:c349276-349004 [Vibrio cholerae M66-2]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT23705 NC_012580:c349276-349004 [Vibrio cholerae M66-2]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
AlphaFold DB A0A0F2IC79

References