Detailed information of TA system TA237048

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1028275..1029811
• Replicon: chromosome
• Accession: NZ_CP092733
• Organism: Comamonas thiooxydans strain CHJ01

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: -
• Locus tag: MJ608_RS04805
• Protein ID: WP_256630440.1
• Coordinates: 1028513..1029811 (+)
• Length: 433 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: MJ608_RS04800
• Protein ID: WP_012838313.1
• Coordinates: 1028275..1028523 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ608_RS04780 (MJ608_04765)	1023557..1025053	+	1497	Protein_925	dihydrolipoyllysine-residue acetyltransferase	-
MJ608_RS04785 (MJ608_04770)	1025169..1025726	+	558	WP_256630438.1	hypothetical protein	-
MJ608_RS04790 (MJ608_04775)	1025942..1027780	+	1839	WP_256630439.1	dihydrolipoyl dehydrogenase	-
MJ608_RS04795 (MJ608_04780)	1027945..1028103	+	159	WP_041744108.1	hypothetical protein	-
MJ608_RS04800 (MJ608_04785)	1028275..1028523	+	249	WP_012838313.1	helix-turn-helix transcriptional regulator	Antitoxin
MJ608_RS04805 (MJ608_04790)	1028513..1029811	+	1299	WP_256630440.1	type II toxin-antitoxin system HipA family toxin	Toxin
MJ608_RS04810 (MJ608_04795)	1030045..1030521	+	477	WP_080692925.1	GFA family protein	-
MJ608_RS04815 (MJ608_04800)	1030654..1032024	-	1371	WP_012838316.1	MFS transporter	-
MJ608_RS04820 (MJ608_04805)	1032800..1033156	-	357	WP_034362530.1	zinc ribbon domain-containing protein YjdM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	947395..1355908	408513

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-60)

Sequences

Toxin

Protein

• Length: 433 a.a.
• Molecular weight: 46957.02 Da
• Isoelectric Point: 7.5323

>T237048 WP_256630440.1 NZ_CP092733:1028513-1029811 [Comamonas thiooxydans]
MTHELEVWLFDGRVGSLSLVHGRLSFAYATDWLALPQATALSQSLPLQAKPFDDHQTRPFFAGLLPEGQMRRLIAQQFQV
SGQNDFALLDHIGGECAGAVTFLEPGQALPQTNGAGATGAPGDVEWLSDQKLLALLDELPRRPMLAGDDGLRLSLAGAQD
KLPVVVDESIPGAVRIGLPRNGAPSSHILKPAIHAVEDSVANEGFCMLLAQVMGLRPANARIQSVLGRSFLLVKRYDREL
NAQDLHQPQLQRLHQEDFCQALGVVPELKYQNEGGPGLAQCFALLRSVTRPSAPQVLRLLDGAIFNALIGNHDAHGKNFS
LLYGGRAPVLAPFYDLLATAIYPQLTPKMAMKIGSKYKFSELEARHWEQFAEEAGLAKAATRKRLQQLANELPAAARKLQ
AAPPHGFAGNAVVTQIVQLIEQRCALTLRRLA

Nucleotide

• Length: 1299 bp

>T237048 NZ_LR778147:4373606-4374148 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCACATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCACATGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAGCCTTCAGAGATTGGTGTTGTCAGATTAGTCATGTTGGGGGTGGCGCG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCGATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8529.72 Da
• Isoelectric Point: 5.6051

>AT237048 WP_012838313.1 NZ_CP092733:1028275-1028523 [Comamonas thiooxydans]
MSTIQTTEQLGAALRTARKQLGLTQPQLALAAGVGTRFIVDLEAGKPTVRLEHVLRVIDALGGELQLGGLPNTASEGADH
DA

Nucleotide

• Length: 249 bp

>AT237048 NZ_LR778147:4373311-4373622 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGTCCCGA
CGCCAAAAACAGACTTCGTGAAGCTGCGCAAGCCGTTGGGTTAGATTTAAGCGCCTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGCGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCC
GAACCTGCTCAACCGACGCTCGCACTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA