Detailed information of TA system TA237047

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 7857..8437
• Replicon: plasmid unnamed2
• Accession: NZ_CP092719
• Organism: Klebsiella pneumoniae strain HvKp-su1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: MKX18_RS28060
• Protein ID: WP_071177730.1
• Coordinates: 8123..8437 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: MKX18_RS28055
• Protein ID: WP_000093040.1
• Coordinates: 7857..8135 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKX18_RS28025 (MKX18_28030)	3917..4066	-	150	WP_032440454.1	colicin release lysis protein	-
MKX18_RS28030 (MKX18_28035)	4151..4408	-	258	WP_032440455.1	colicin E3-like toxin immunity protein	-
MKX18_RS28035 (MKX18_28040)	4418..6103	-	1686	WP_032440457.1	colicin-like bacteriocin tRNase domain-containing protein	-
MKX18_RS28040 (MKX18_28045)	6430..6675	+	246	WP_032440458.1	hypothetical protein	-
MKX18_RS28045 (MKX18_28050)	6949..7320	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
MKX18_RS28050 (MKX18_28055)	7317..7682	+	366	WP_072354022.1	TonB family protein	-
MKX18_RS28055 (MKX18_28060)	7857..8135	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
MKX18_RS28060 (MKX18_28065)	8123..8437	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MKX18_RS28065 (MKX18_28070)	8601..9029	+	429	WP_001140599.1	hypothetical protein	-
MKX18_RS28070 (MKX18_28075)	9055..9234	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
MKX18_RS28075 (MKX18_28080)	9261..9791	-	531	WP_071177729.1	hypothetical protein	-
MKX18_RS28080 (MKX18_28085)	9798..10058	-	261	WP_228733410.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10061	10061

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T237047 WP_071177730.1 NZ_CP092719:8123-8437 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T237047 NZ_LR778147:4338911-4339129 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT237047 WP_000093040.1 NZ_CP092719:7857-8135 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT237047 NZ_LR778147:4338511-4338885 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1