Detailed information of TA system TA237046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 112061..112704
• Replicon: plasmid unnamed1
• Accession: NZ_CP092718
• Organism: Klebsiella pneumoniae strain HvKp-su1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: MKX18_RS27875
• Protein ID: WP_001044770.1
• Coordinates: 112061..112477 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: MKX18_RS27880
• Protein ID: WP_001261282.1
• Coordinates: 112474..112704 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKX18_RS27855 (107258)	107258..108139	-	882	WP_004199234.1	carbapenem-hydrolyzing class A beta-lactamase KPC-2	-
MKX18_RS27865 (109418)	109418..110440	-	1023	WP_000361404.1	helicase UvrD	-
MKX18_RS27870 (110425)	110425..111987	-	1563	WP_004206609.1	AAA family ATPase	-
MKX18_RS27875 (112061)	112061..112477	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
MKX18_RS27880 (112474)	112474..112704	-	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MKX18_RS27885 (112661)	112661..113122	+	462	WP_014343465.1	hypothetical protein	-
MKX18_RS27890 (113283)	113283..114227	+	945	WP_011977810.1	hypothetical protein	-
MKX18_RS27895 (114264)	114264..114656	+	393	WP_011977811.1	hypothetical protein	-
MKX18_RS27900 (114714)	114714..115235	+	522	WP_013214008.1	hypothetical protein	-
MKX18_RS27905 (115281)	115281..115484	+	204	WP_011977813.1	hypothetical protein	-
MKX18_RS27910 (115514)	115514..116518	+	1005	WP_011977814.1	hypothetical protein	-
MKX18_RS27915 (116702)	116702..117481	+	780	WP_013214009.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..133346	133346

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T237046 WP_001044770.1 NZ_CP092718:c112477-112061 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T237046 NZ_LR778147:3936526-3936912 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT237046 WP_001261282.1 NZ_CP092718:c112704-112474 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT237046 NZ_LR778147:3936902-3937231 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3