Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 112061..112704 Replicon plasmid unnamed1
Accession NZ_CP092718
Organism Klebsiella pneumoniae strain HvKp-su1

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag MKX18_RS27875 Protein ID WP_001044770.1
Coordinates 112061..112477 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag MKX18_RS27880 Protein ID WP_001261282.1
Coordinates 112474..112704 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKX18_RS27855 (107258) 107258..108139 - 882 WP_004199234.1 carbapenem-hydrolyzing class A beta-lactamase KPC-2 -
MKX18_RS27865 (109418) 109418..110440 - 1023 WP_000361404.1 helicase UvrD -
MKX18_RS27870 (110425) 110425..111987 - 1563 WP_004206609.1 AAA family ATPase -
MKX18_RS27875 (112061) 112061..112477 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
MKX18_RS27880 (112474) 112474..112704 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MKX18_RS27885 (112661) 112661..113122 + 462 WP_014343465.1 hypothetical protein -
MKX18_RS27890 (113283) 113283..114227 + 945 WP_011977810.1 hypothetical protein -
MKX18_RS27895 (114264) 114264..114656 + 393 WP_011977811.1 hypothetical protein -
MKX18_RS27900 (114714) 114714..115235 + 522 WP_013214008.1 hypothetical protein -
MKX18_RS27905 (115281) 115281..115484 + 204 WP_011977813.1 hypothetical protein -
MKX18_RS27910 (115514) 115514..116518 + 1005 WP_011977814.1 hypothetical protein -
MKX18_RS27915 (116702) 116702..117481 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2 - 1..133346 133346


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T237046 WP_001044770.1 NZ_CP092718:c112477-112061 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T237046 NZ_LR778147:3936526-3936912 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT237046 WP_001261282.1 NZ_CP092718:c112704-112474 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT237046 NZ_LR778147:3936902-3937231 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References