Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | parDE/ParE-Phd |
| Location | 345306..345884 | Replicon | chromosome II |
| Accession | NC_012580 | ||
| Organism | Vibrio cholerae M66-2 | ||
Toxin (Protein)
| Gene name | parE | Uniprot ID | O68848 |
| Locus tag | VCM66_RS14930 | Protein ID | WP_001180243.1 |
| Coordinates | 345306..345623 (-) | Length | 106 a.a. |
Antitoxin (Protein)
| Gene name | parD | Uniprot ID | Q7DCR7 |
| Locus tag | VCM66_RS14935 | Protein ID | WP_000557292.1 |
| Coordinates | 345642..345884 (-) | Length | 81 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| VCM66_RS14895 | 340830..341012 | + | 183 | WP_000923340.1 | DUF645 family protein | - |
| VCM66_RS14905 | 341366..341548 | + | 183 | WP_000923153.1 | DUF645 family protein | - |
| VCM66_RS14910 | 341743..342420 | + | 678 | WP_000254617.1 | HNH endonuclease | - |
| VCM66_RS14915 | 342582..343790 | + | 1209 | WP_000272282.1 | deoxyguanosinetriphosphate triphosphohydrolase family protein | - |
| VCM66_RS14920 | 343952..344656 | + | 705 | WP_000087610.1 | HNH endonuclease | - |
| VCM66_RS14925 | 344814..345152 | + | 339 | WP_000713853.1 | hypothetical protein | - |
| VCM66_RS14930 | 345306..345623 | - | 318 | WP_001180243.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| VCM66_RS14935 | 345642..345884 | - | 243 | WP_000557292.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| VCM66_RS14940 | 346128..346514 | + | 387 | WP_001015867.1 | NUDIX hydrolase | - |
| VCM66_RS18770 | 346571..346684 | + | 114 | WP_001900214.1 | hypothetical protein | - |
| VCM66_RS14945 | 346633..346914 | + | 282 | WP_001894429.1 | DUF1289 domain-containing protein | - |
| VCM66_RS14955 | 347172..347708 | + | 537 | WP_000469482.1 | GNAT family N-acetyltransferase | - |
| VCM66_RS14960 | 347845..348360 | + | 516 | WP_001201509.1 | lipocalin family protein | - |
| VCM66_RS14965 | 348510..349007 | - | 498 | WP_000982260.1 | GNAT family N-acetyltransferase | - |
| VCM66_RS14970 | 349004..349276 | - | 273 | WP_000246253.1 | DUF1778 domain-containing protein | - |
| VCM66_RS18790 | 349669..349794 | + | 126 | WP_001900195.1 | DUF645 family protein | - |
| VCM66_RS19320 | 349810..349848 | + | 39 | WP_106019118.1 | hypothetical protein | - |
| VCM66_RS14980 | 350044..350490 | + | 447 | WP_000006157.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 305869..409778 | 103909 | |
| - | inside | Integron | - | - | 310949..409402 | 98453 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 106 a.a. Molecular weight: 12158.97 Da Isoelectric Point: 9.7495
>T23704 WP_001180243.1 NC_012580:c345623-345306 [Vibrio cholerae M66-2]
MQNKQYKLSQLAQEHLLKIKHYTIENFAEAQWQKYKSTLLSGFQTLADNPGLGKSCEDIYQNGFYFPVGKHMAYYTKEAN
FILIVAVLGQSQLPQKHLKQSRFVS
MQNKQYKLSQLAQEHLLKIKHYTIENFAEAQWQKYKSTLLSGFQTLADNPGLGKSCEDIYQNGFYFPVGKHMAYYTKEAN
FILIVAVLGQSQLPQKHLKQSRFVS
Download Length: 318 bp
>T23704 NC_012580:c345623-345306 [Vibrio cholerae M66-2]
ATGCAAAATAAACAATATAAGCTCAGCCAATTAGCCCAAGAGCACTTACTCAAGATTAAACACTACACCATTGAAAATTT
CGCTGAAGCGCAGTGGCAAAAATATAAGTCGACCTTGCTTTCAGGTTTCCAAACTCTTGCGGATAACCCAGGACTAGGAA
AAAGCTGTGAAGATATTTACCAAAATGGTTTTTACTTTCCAGTGGGGAAACACATGGCTTATTACACCAAAGAAGCAAAC
TTCATTCTCATTGTTGCGGTATTAGGGCAATCACAACTGCCCCAAAAGCATCTCAAACAGTCACGCTTTGTTTCTTAG
ATGCAAAATAAACAATATAAGCTCAGCCAATTAGCCCAAGAGCACTTACTCAAGATTAAACACTACACCATTGAAAATTT
CGCTGAAGCGCAGTGGCAAAAATATAAGTCGACCTTGCTTTCAGGTTTCCAAACTCTTGCGGATAACCCAGGACTAGGAA
AAAGCTGTGAAGATATTTACCAAAATGGTTTTTACTTTCCAGTGGGGAAACACATGGCTTATTACACCAAAGAAGCAAAC
TTCATTCTCATTGTTGCGGTATTAGGGCAATCACAACTGCCCCAAAAGCATCTCAAACAGTCACGCTTTGTTTCTTAG
Antitoxin
Download Length: 81 a.a. Molecular weight: 8961.25 Da Isoelectric Point: 5.6630
>AT23704 WP_000557292.1 NC_012580:c345884-345642 [Vibrio cholerae M66-2]
MHTLTANDAKRNFGELLLSAQREPVIISKNSKNTVVVMSIKDFEELEAMKLDYLKHCFESAQKDLDSGKTVDGATFLNTL
MHTLTANDAKRNFGELLLSAQREPVIISKNSKNTVVVMSIKDFEELEAMKLDYLKHCFESAQKDLDSGKTVDGATFLNTL
Download Length: 243 bp
>AT23704 NC_012580:c345884-345642 [Vibrio cholerae M66-2]
ATGCACACACTAACAGCAAATGATGCTAAACGAAATTTTGGTGAACTTCTTCTAAGCGCACAACGCGAACCTGTCATCAT
CAGCAAAAACAGTAAGAATACTGTCGTTGTCATGTCCATTAAGGACTTTGAAGAACTTGAAGCAATGAAACTCGATTATC
TCAAACACTGCTTTGAGTCTGCTCAGAAAGACTTAGATAGTGGCAAAACCGTTGATGGTGCCACTTTTCTAAACACCCTT
TGA
ATGCACACACTAACAGCAAATGATGCTAAACGAAATTTTGGTGAACTTCTTCTAAGCGCACAACGCGAACCTGTCATCAT
CAGCAAAAACAGTAAGAATACTGTCGTTGTCATGTCCATTAAGGACTTTGAAGAACTTGAAGCAATGAAACTCGATTATC
TCAAACACTGCTTTGAGTCTGCTCAGAAAGACTTAGATAGTGGCAAAACCGTTGATGGTGCCACTTTTCTAAACACCCTT
TGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0K9UJR8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q7DCR7 |