Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5436918..5437543 Replicon chromosome
Accession NZ_CP092717
Organism Klebsiella pneumoniae strain HvKp-su1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MKX18_RS26775 Protein ID WP_002882817.1
Coordinates 5436918..5437301 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MKX18_RS26780 Protein ID WP_004150355.1
Coordinates 5437301..5437543 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKX18_RS26760 (MKX18_26755) 5434284..5435186 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MKX18_RS26765 (MKX18_26760) 5435183..5435818 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MKX18_RS26770 (MKX18_26765) 5435815..5436744 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MKX18_RS26775 (MKX18_26770) 5436918..5437301 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MKX18_RS26780 (MKX18_26775) 5437301..5437543 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MKX18_RS26785 (MKX18_26780) 5437748..5438665 + 918 WP_002882812.1 alpha/beta hydrolase -
MKX18_RS26790 (MKX18_26785) 5438679..5439620 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MKX18_RS26795 (MKX18_26790) 5439665..5440102 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MKX18_RS26800 (MKX18_26795) 5440099..5440959 - 861 WP_002882807.1 virulence factor BrkB family protein -
MKX18_RS26805 (MKX18_26800) 5440953..5441552 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T237039 WP_002882817.1 NZ_CP092717:c5437301-5436918 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T237039 NZ_LR778147:2574871-2575122 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCCCTGGAGGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGGTAGCCTGAAATCTGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT237039 WP_004150355.1 NZ_CP092717:c5437543-5437301 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT237039 NZ_LR778147:2575119-2575373 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References