Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4758440..4759250 Replicon chromosome
Accession NZ_CP092717
Organism Klebsiella pneumoniae strain HvKp-su1

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag MKX18_RS23480 Protein ID WP_002887280.1
Coordinates 4758440..4758973 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag MKX18_RS23485 Protein ID WP_002887278.1
Coordinates 4758984..4759250 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKX18_RS23475 (MKX18_23475) 4757271..4758392 + 1122 WP_002887282.1 cupin domain-containing protein -
MKX18_RS23480 (MKX18_23480) 4758440..4758973 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
MKX18_RS23485 (MKX18_23485) 4758984..4759250 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
MKX18_RS23490 (MKX18_23490) 4759353..4760786 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
MKX18_RS23495 (MKX18_23495) 4760776..4761459 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
MKX18_RS23500 (MKX18_23500) 4761631..4763016 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
MKX18_RS23505 (MKX18_23505) 4763034..4763378 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T237036 WP_002887280.1 NZ_CP092717:c4758973-4758440 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T237036 NZ_LR778147:c1340078-1339701 [Escherichia coli]
ATGAAAACATTACCCGACACACACATACGGGAGGCATCGCGCTGCCCGTGTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCGGCACTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGATGCGGTGAACGTTCTCGTTGAAAAATACGCGCTGGTGCGTACCGACCAGTCCGGGTTC
AGCATTTGTCCCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCAACAGGTCTGATGACCCGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT237036 WP_002887278.1 NZ_CP092717:c4759250-4758984 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT237036 NZ_LR778147:c1340536-1340168 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGTGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCAGATTCGTACCACCTGGACCAGGCCTTTCCGCTACTGATGAAACAACTGGAACTCATGCTCACCAGTGGT
GAACTAAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAACGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References