Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/ParE-RHH
Location 321980..322518 Replicon chromosome II
Accession NC_012580
Organism Vibrio cholerae M66-2

Toxin (Protein)


Gene name parE Uniprot ID Q9KMJ0
Locus tag VCM66_RS14765 Protein ID WP_000802136.1
Coordinates 321980..322279 (-) Length 100 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID P58093
Locus tag VCM66_RS14770 Protein ID WP_001107719.1
Coordinates 322276..322518 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VCM66_RS14720 317111..317587 + 477 WP_001047183.1 GNAT family N-acetyltransferase -
VCM66_RS14730 317883..318062 + 180 WP_001889238.1 DUF645 family protein -
VCM66_RS14735 318256..318771 + 516 WP_000343784.1 GNAT family N-acetyltransferase -
VCM66_RS14740 318907..319422 + 516 WP_001201509.1 lipocalin family protein -
VCM66_RS14745 319565..320101 + 537 WP_000644491.1 nucleotidyltransferase family protein -
VCM66_RS18685 320570..320680 + 111 WP_071908335.1 DUF3265 domain-containing protein -
VCM66_RS14750 320893..321045 + 153 WP_001884520.1 DUF645 family protein -
VCM66_RS14755 321245..321523 - 279 WP_000578476.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family -
VCM66_RS14760 321520..321804 - 285 WP_000381183.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
VCM66_RS14765 321980..322279 - 300 WP_000802136.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
VCM66_RS14770 322276..322518 - 243 WP_001107719.1 type II toxin-antitoxin system ParD family antitoxin Antitoxin
VCM66_RS18700 322937..323014 + 78 WP_071908337.1 DUF645 family protein -
VCM66_RS19545 323018..323044 + 27 Protein_277 hypothetical protein -
VCM66_RS18715 323630..323773 + 144 WP_001900215.1 hypothetical protein -
VCM66_RS19260 323867..324046 + 180 Protein_279 DUF645 family protein -
VCM66_RS14780 324264..324692 + 429 WP_000620002.1 N-acetyltransferase -
VCM66_RS14785 325280..325762 + 483 WP_000422940.1 GNAT family N-acetyltransferase -
VCM66_RS14795 326570..326839 + 270 WP_001114075.1 DUF4160 domain-containing protein -
VCM66_RS14800 326833..327354 + 522 WP_000921692.1 DUF2442 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 305869..409778 103909
- inside Integron - - 310949..409402 98453


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-92)

Antitoxin

Antitoxin ParD

(1-79)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11589.29 Da        Isoelectric Point: 9.9232

>T23701 WP_000802136.1 NC_012580:c322279-321980 [Vibrio cholerae M66-2]
MKPFNLTVAAKADLRDIALFTQRRWGKEQRNVYLKQFDDSFWLLAENPDIGKSCDEIREGYRKFPQGSHVIFYQQTGSQQ
IRVIRILHKSMDVNPIFGA

Download         Length: 300 bp

>T23701 NC_012580:c322279-321980 [Vibrio cholerae M66-2]
ATGAAACCATTTAATCTTACCGTCGCCGCCAAAGCCGATTTACGTGATATTGCTTTATTCACTCAACGACGCTGGGGAAA
AGAGCAGCGAAATGTTTATTTAAAGCAATTCGATGATTCCTTTTGGCTTTTAGCGGAAAATCCCGACATTGGTAAATCAT
GCGATGAAATCCGAGAGGGATACAGAAAATTTCCCCAAGGGAGTCACGTCATCTTTTATCAGCAAACCGGCAGCCAACAA
ATCCGGGTGATCCGAATTCTTCATAAGAGCATGGATGTGAACCCAATATTCGGCGCATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8964.84 Da        Isoelectric Point: 4.1677

>AT23701 WP_001107719.1 NC_012580:c322518-322276 [Vibrio cholerae M66-2]
MAKNTSITLGEHFDGFITSQIQSGRYGSASEVIRSALRLLENQETKLQSLRQLLIEGEQSGDADYDLDSFINELDSENIR

Download         Length: 243 bp

>AT23701 NC_012580:c322518-322276 [Vibrio cholerae M66-2]
ATGGCTAAAAATACAAGTATCACTCTTGGTGAACACTTCGATGGCTTTATTACAAGCCAAATACAAAGTGGGCGTTACGG
CTCAGCAAGTGAAGTCATTCGCTCTGCGCTACGTCTACTCGAAAACCAAGAAACCAAACTACAGTCACTCCGTCAACTAC
TTATTGAAGGAGAGCAAAGTGGTGACGCTGATTATGACCTTGATAGCTTCATCAATGAACTCGATAGTGAAAACATTCGA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7R5A

Antitoxin

Source ID Structure
PDB 7R5A
PDB 7B22
AlphaFold DB A0A151JFU4

References