Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4352531..4353028 Replicon chromosome
Accession NZ_CP092702
Organism Escherichia coli strain S-P-C-029.01

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MJ592_RS21090 Protein ID WP_001173343.1
Coordinates 4352855..4353028 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag MJ592_RS21085 Protein ID WP_000398619.1
Coordinates 4352531..4352827 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ592_RS21065 (4349262) 4349262..4349981 - 720 WP_000611288.1 two-component system response regulator DcuR -
MJ592_RS21070 (4349978) 4349978..4351609 - 1632 WP_001216477.1 sensor histidine kinase -
MJ592_RS21075 (4351790) 4351790..4352020 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
MJ592_RS21080 (4352032) 4352032..4352304 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
MJ592_RS21085 (4352531) 4352531..4352827 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MJ592_RS21090 (4352855) 4352855..4353028 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MJ592_RS21095 (4353147) 4353147..4354664 - 1518 WP_001295074.1 lysine--tRNA ligase -
MJ592_RS21100 (4354901) 4354901..4356358 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T237000 WP_001173343.1 NZ_CP092702:4352855-4353028 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T237000 NZ_LR778145:1376637-1376972 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGATCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCGGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT237000 WP_000398619.1 NZ_CP092702:4352531-4352827 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT237000 NZ_LR778145:1376389-1376637 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References