Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RelE-RelB |
| Location | 321245..321804 | Replicon | chromosome II |
| Accession | NC_012580 | ||
| Organism | Vibrio cholerae M66-2 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | Q9K2M8 |
| Locus tag | VCM66_RS14755 | Protein ID | WP_000578476.1 |
| Coordinates | 321245..321523 (-) | Length | 93 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | Q9L991 |
| Locus tag | VCM66_RS14760 | Protein ID | WP_000381183.1 |
| Coordinates | 321520..321804 (-) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| VCM66_RS14720 | 317111..317587 | + | 477 | WP_001047183.1 | GNAT family N-acetyltransferase | - |
| VCM66_RS14730 | 317883..318062 | + | 180 | WP_001889238.1 | DUF645 family protein | - |
| VCM66_RS14735 | 318256..318771 | + | 516 | WP_000343784.1 | GNAT family N-acetyltransferase | - |
| VCM66_RS14740 | 318907..319422 | + | 516 | WP_001201509.1 | lipocalin family protein | - |
| VCM66_RS14745 | 319565..320101 | + | 537 | WP_000644491.1 | nucleotidyltransferase family protein | - |
| VCM66_RS18685 | 320570..320680 | + | 111 | WP_071908335.1 | DUF3265 domain-containing protein | - |
| VCM66_RS14750 | 320893..321045 | + | 153 | WP_001884520.1 | DUF645 family protein | - |
| VCM66_RS14755 | 321245..321523 | - | 279 | WP_000578476.1 | type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family | Toxin |
| VCM66_RS14760 | 321520..321804 | - | 285 | WP_000381183.1 | type II toxin-antitoxin system RelB/DinJ family antitoxin | Antitoxin |
| VCM66_RS14765 | 321980..322279 | - | 300 | WP_000802136.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| VCM66_RS14770 | 322276..322518 | - | 243 | WP_001107719.1 | type II toxin-antitoxin system ParD family antitoxin | - |
| VCM66_RS18700 | 322937..323014 | + | 78 | WP_071908337.1 | DUF645 family protein | - |
| VCM66_RS19545 | 323018..323044 | + | 27 | Protein_277 | hypothetical protein | - |
| VCM66_RS18715 | 323630..323773 | + | 144 | WP_001900215.1 | hypothetical protein | - |
| VCM66_RS19260 | 323867..324046 | + | 180 | Protein_279 | DUF645 family protein | - |
| VCM66_RS14780 | 324264..324692 | + | 429 | WP_000620002.1 | N-acetyltransferase | - |
| VCM66_RS14785 | 325280..325762 | + | 483 | WP_000422940.1 | GNAT family N-acetyltransferase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 305869..409778 | 103909 | |
| - | inside | Integron | - | - | 310949..409402 | 98453 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 93 a.a. Molecular weight: 10819.59 Da Isoelectric Point: 5.1651
>T23700 WP_000578476.1 NC_012580:c321523-321245 [Vibrio cholerae M66-2]
MIFWEEASLNDREKIFEFLYDFNPAAAKKTDELIEAKVENLLEQPLIGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPND
MIFWEEASLNDREKIFEFLYDFNPAAAKKTDELIEAKVENLLEQPLIGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPND
Download Length: 279 bp
>T23700 NC_012580:c321523-321245 [Vibrio cholerae M66-2]
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCGGC
TAAAAAAACGGATGAGCTCATAGAAGCCAAAGTCGAAAATTTGCTTGAGCAACCACTAATCGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTGCTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGTGTACTACATCAGAAACAAAAATTTCCAAACGACTGA
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCGGC
TAAAAAAACGGATGAGCTCATAGAAGCCAAAGTCGAAAATTTGCTTGAGCAACCACTAATCGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTGCTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGTGTACTACATCAGAAACAAAAATTTCCAAACGACTGA
Antitoxin
Download Length: 95 a.a. Molecular weight: 10901.25 Da Isoelectric Point: 8.0775
>AT23700 WP_000381183.1 NC_012580:c321804-321520 [Vibrio cholerae M66-2]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDAWLTEQVNQAFEKFDSGKAVFIEHDIAKAR
MAERKAKIRNRGHA
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDAWLTEQVNQAFEKFDSGKAVFIEHDIAKAR
MAERKAKIRNRGHA
Download Length: 285 bp
>AT23700 NC_012580:c321804-321520 [Vibrio cholerae M66-2]
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTTAGCGATGCTTGCCGTGAACTCACTGAACAATTAGCTGAGCAGCAACGCAAGGCATTATCTCACGATGCATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTTAGCGATGCTTGCCGTGAACTCACTGAACAATTAGCTGAGCAGCAACGCAAGGCATTATCTCACGATGCATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q9K2M8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A067BL33 |