236991

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 2765897..2767334 Replicon chromosome
Accession NZ_CP092702
Organism Escherichia coli strain S-P-C-029.01

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag MJ592_RS13550 Protein ID WP_000155570.1
Coordinates 2765897..2766970 (+) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag MJ592_RS13555 Protein ID WP_000461704.1
Coordinates 2766963..2767334 (+) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ592_RS13535 (2761330) 2761330..2763519 - 2190 WP_000136402.1 DEAD/DEAH box helicase -
MJ592_RS13540 (2763516) 2763516..2765132 - 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
MJ592_RS13545 (2765492) 2765492..2765755 - 264 WP_000502050.1 DUF1524 domain-containing protein -
MJ592_RS13550 (2765897) 2765897..2766970 + 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
MJ592_RS13555 (2766963) 2766963..2767334 + 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
MJ592_RS13560 (2767689) 2767689..2768552 + 864 WP_000787597.1 GTPase family protein -
MJ592_RS13565 (2768644) 2768644..2769465 + 822 WP_000197391.1 DUF932 domain-containing protein -
MJ592_RS13570 (2769682) 2769682..2770383 + 702 WP_000189409.1 WYL domain-containing protein -
MJ592_RS13575 (2770424) 2770424..2770660 + 237 WP_001144029.1 protein YpjK -
MJ592_RS13580 (2770660) 2770660..2771103 + 444 WP_000824222.1 lipoprotein YfjS -
MJ592_RS13585 (2771127) 2771127..2771594 + 468 WP_000702495.1 protein YfjT -
MJ592_RS13590 (2771981) 2771981..2772133 - 153 Protein_2668 arsenate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T236991 WP_000155570.1 NZ_CP092702:2765897-2766970 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T236991 NZ_LR778143:3616171-3616274 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT236991 WP_000461704.1 NZ_CP092702:2766963-2767334 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT236991 NZ_LR778143:c3616312-3616041 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCTTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References