Detailed information of TA system TA023697

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapB(antitoxin)
• Location: 4732136..4732719
• Replicon: chromosome
• Accession: NC_012560
• Organism: Azotobacter vinelandii DJ

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: M9YMQ6
• Locus tag: AVIN_RS21290
• Protein ID: WP_012703131.1
• Coordinates: 4732339..4732719 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M9YNF6
• Locus tag: AVIN_RS21285
• Protein ID: WP_012703130.1
• Coordinates: 4732136..4732342 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVIN_RS21265	4727815..4729149	+	1335	WP_012703124.1	HAMP domain-containing protein	-
AVIN_RS21270	4729493..4730251	+	759	WP_012703126.1	TorF family putative porin	-
AVIN_RS21275	4730506..4731303	-	798	WP_012703127.1	4-hydroxy-2-oxoheptanedioate aldolase	-
AVIN_RS21280	4731449..4731901	+	453	WP_012703128.1	GNAT family N-acetyltransferase	-
AVIN_RS21285	4732136..4732342	+	207	WP_012703130.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
AVIN_RS21290	4732339..4732719	+	381	WP_012703131.1	PIN domain-containing protein	Toxin
AVIN_RS21295	4733112..4734590	-	1479	WP_012703132.1	anthranilate synthase component I	-
AVIN_RS21300	4734777..4735430	-	654	WP_072432469.1	phosphoglycolate phosphatase	-
AVIN_RS21305	4735475..4736149	-	675	WP_012703134.1	ribulose-phosphate 3-epimerase	-
AVIN_RS21310	4736247..4737527	-	1281	WP_012703135.1	4-aminobutyrate--2-oxoglutarate transaminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-114)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-43)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13873.21 Da
• Isoelectric Point: 6.7009

>T23697 WP_012703131.1 NC_012560:4732339-4732719 [Azotobacter vinelandii DJ]
MKVLVDTSVWIDHFRNGNAALVELLGSDDALTHPMVIAEIACGTPPAPRAQTLDNLGLLQSCRQATLQEVMTFIEREKLY
GLGCGLVDIALLASTLITPGTLLWTLDKRLASLARRFDVAYRPMRH

Nucleotide

• Length: 381 bp

>T23697 NC_012560:4732339-4732719 [Azotobacter vinelandii DJ]
ATGAAAGTGCTGGTCGACACATCGGTGTGGATCGACCACTTTCGCAATGGCAACGCCGCGCTGGTCGAACTGCTCGGCTC
GGACGACGCCCTGACCCACCCCATGGTCATCGCCGAAATCGCCTGTGGTACACCGCCGGCTCCACGCGCCCAGACACTGG
ATAACCTGGGGCTGTTGCAATCCTGCAGACAGGCGACCCTGCAGGAAGTCATGACGTTCATCGAGCGGGAAAAACTCTAT
GGCCTGGGGTGCGGGCTGGTGGATATAGCTCTGCTGGCATCGACGCTCATCACGCCGGGGACACTGCTATGGACGCTGGA
CAAGCGCCTGGCAAGCCTGGCTCGGCGTTTCGATGTCGCCTATCGCCCGATGAGGCACTAG

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7703.86 Da
• Isoelectric Point: 10.5812

>AT23697 WP_012703130.1 NC_012560:4732136-4732342 [Azotobacter vinelandii DJ]
MRTTVTIDDALYEKALEMADPGMDKADIFREAMKTFVRVQAARRLAALGGTTPNVRNVPRRRERAEPQ

Nucleotide

• Length: 207 bp

>AT23697 NC_012560:4732136-4732342 [Azotobacter vinelandii DJ]
ATGCGCACCACCGTCACTATCGATGACGCGCTCTATGAAAAGGCTCTCGAAATGGCCGATCCGGGCATGGACAAGGCGGA
CATCTTCCGCGAGGCCATGAAGACCTTCGTGCGGGTTCAAGCCGCCAGACGCCTCGCGGCGCTGGGAGGAACCACGCCGA
ACGTCCGGAACGTGCCTCGCCGTCGGGAGAGAGCCGAGCCGCAATGA

Structures

Toxin

• AlphaFold DB: M9YMQ6

Antitoxin

• AlphaFold DB: M9YNF6