Detailed information of TA system TA023695

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 4106572..4107180
• Replicon: chromosome
• Accession: NC_012560
• Organism: Azotobacter vinelandii DJ

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: M9YR94
• Locus tag: AVIN_RS18510
• Protein ID: WP_012702568.1
• Coordinates: 4106890..4107180 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: AVIN_RS18505
• Protein ID: WP_012702567.1
• Coordinates: 4106572..4106880 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVIN_RS26225	4101753..4102091	+	339	WP_144061665.1	hypothetical protein	-
AVIN_RS18465	4102343..4103233	-	891	Protein_3701	IS3-like element ISAzvi9 family transposase	-
AVIN_RS18475	4103307..4104172	+	866	Protein_3702	IS110 family transposase	-
AVIN_RS18480	4104426..4104689	-	264	Protein_3703	transposase	-
AVIN_RS18485	4104900..4105124	+	225	WP_012702563.1	AlpA family phage regulatory protein	-
AVIN_RS18490	4105126..4105509	+	384	WP_012702564.1	hypothetical protein	-
AVIN_RS18495	4105506..4106177	+	672	WP_012702565.1	Rha family transcriptional regulator	-
AVIN_RS18500	4106174..4106422	+	249	WP_012702566.1	hypothetical protein	-
AVIN_RS18505	4106572..4106880	-	309	WP_012702567.1	putative addiction module antidote protein	Antitoxin
AVIN_RS18510	4106890..4107180	-	291	WP_012702568.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AVIN_RS26595	4107535..4107708	+	174	WP_012702569.1	hypothetical protein	-
AVIN_RS18515	4107705..4107959	+	255	WP_012702570.1	hypothetical protein	-
AVIN_RS18520	4107956..4108255	+	300	WP_012702571.1	hypothetical protein	-
AVIN_RS26235	4108303..4108668	+	366	WP_012702573.1	hypothetical protein	-
AVIN_RS18530	4108747..4109133	+	387	WP_012702575.1	toprim domain-containing protein	-
AVIN_RS18535	4109130..4109522	+	393	WP_012702576.1	hypothetical protein	-
AVIN_RS18540	4110226..4110429	+	204	WP_012702577.1	hypothetical protein	-
AVIN_RS18545	4110924..4111133	+	210	WP_012702578.1	hypothetical protein	-
AVIN_RS18550	4111249..4111485	-	237	WP_012702579.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4100541..4116056	15515

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-92)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10796.24 Da
• Isoelectric Point: 8.0567

>T23695 WP_012702568.1 NC_012560:c4107180-4106890 [Azotobacter vinelandii DJ]
MKSILTTDVFDRWFSGLRDRRAAVRVQARIDRAEDGNFGDCEPVGEGVSEMRIHYGPGYRVYFTQRGHELVILLAGGDKS
SQAKDIKAAIDMARHL

Nucleotide

• Length: 291 bp

>T23695 NC_012560:c4107180-4106890 [Azotobacter vinelandii DJ]
ATGAAAAGCATCCTCACCACGGACGTGTTCGACCGCTGGTTCTCCGGCCTGCGGGACAGGCGGGCGGCAGTGCGAGTACA
GGCCCGCATTGACCGTGCCGAAGATGGCAACTTCGGCGATTGCGAGCCGGTCGGCGAAGGCGTGTCGGAGATGCGCATCC
ACTACGGGCCGGGCTATCGCGTGTATTTCACGCAACGCGGGCACGAGTTGGTCATCCTGCTGGCCGGTGGCGACAAGTCC
AGCCAAGCGAAAGACATCAAGGCCGCTATCGACATGGCGCGGCATCTGTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 10922.51 Da
• Isoelectric Point: 6.4795

>AT23695 WP_012702567.1 NC_012560:c4106880-4106572 [Azotobacter vinelandii DJ]
MTTVKLRKWDSAEHLKTDEDMALYLEACIEEAGDDAAFIAKALGTIARAKGMAQLARDTGLGRESLYKALSGEGNPSFAT
ILKVIQALGLRLHVEAGAAHTH

Nucleotide

• Length: 309 bp

>AT23695 NC_012560:c4106880-4106572 [Azotobacter vinelandii DJ]
ATGACTACCGTCAAACTGCGCAAGTGGGATAGCGCCGAACACCTCAAGACCGATGAGGACATGGCGCTTTATCTTGAGGC
TTGCATCGAGGAGGCTGGCGACGATGCGGCGTTCATCGCCAAGGCTCTCGGCACCATCGCCCGCGCCAAGGGCATGGCCC
AATTGGCACGAGACACCGGCCTCGGGCGCGAAAGTCTGTACAAGGCGCTGTCCGGCGAGGGCAACCCCAGCTTCGCCACC
ATCCTCAAGGTGATACAGGCGCTCGGCCTCCGGCTTCACGTTGAAGCCGGTGCGGCGCACACTCACTGA

Structures

Toxin

• AlphaFold DB: M9YR94