Detailed information of TA system TA236937

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 45553..46900
• Replicon: plasmid pIFL1_3
• Accession: NZ_CP092686
• Organism: Vibrio campbellii strain IFL1

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: MKR81_RS28425
• Protein ID: WP_240307067.1
• Coordinates: 45553..46494 (-)
• Length: 314 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: -
• Locus tag: MKR81_RS28430
• Protein ID: WP_240307068.1
• Coordinates: 46496..46900 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKR81_RS28405 (MKR81_28405)	40975..41460	-	486	WP_240307064.1	hypothetical protein	-
MKR81_RS28410 (MKR81_28410)	41457..42752	-	1296	WP_104036870.1	ATP-binding protein	-
MKR81_RS28415 (MKR81_28415)	42746..44011	-	1266	WP_240307065.1	restriction endonuclease subunit S	-
MKR81_RS28420 (MKR81_28420)	44001..45533	-	1533	WP_240307066.1	type I restriction-modification system subunit M	-
MKR81_RS28425 (MKR81_28425)	45553..46494	-	942	WP_240307067.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
MKR81_RS28430 (MKR81_28430)	46496..46900	-	405	WP_240307068.1	DUF6088 family protein	Antitoxin
MKR81_RS28435 (MKR81_28435)	47089..47682	-	594	WP_085343577.1	tyrosine-type recombinase/integrase	-
MKR81_RS28440 (MKR81_28440)	47962..48204	-	243	WP_061007694.1	helix-turn-helix domain-containing protein	-
MKR81_RS28445 (MKR81_28445)	48784..49236	+	453	WP_021018079.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..77175	77175

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (32-300)

Antitoxin

• Protein of unknown function DUF6088 (7-130)

Sequences

Toxin

Protein

• Length: 314 a.a.
• Molecular weight: 35835.32 Da
• Isoelectric Point: 5.3108

>T236937 WP_240307067.1 NZ_CP092686:c46494-45553 [Vibrio campbellii]
MNTLKQQFLEVSDALELGNPAIIEKDYWVVALLAELAKVSPEHHQMVFSGGTALAKSNVKILRMSEDVDIKLIPNPAFDE
LSRARKKAARKACVNEIEQAITRSELFTVEERSVRDEYRYVEFELKYPQQFSQAPCLRPIIKLELIETIPLLDVESRSIQ
SLVAELYQQPQEVSAFDCVSIHATLVEKIISMLRRTMSVKRNTERNDDVALVRHIYDVHCITKLAMPELNGVVGLFKAVL
EEDIARFGNQHAEFVANPKQELQLGLKELEENPIFRQRFQDFVTPMVFNTEPHDFDTCFASFKRIAASLIEKI

Nucleotide

• Length: 942 bp

>T236937 NZ_LR778141:c1184608-1184321 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 15168.54 Da
• Isoelectric Point: 10.4983

>AT236937 WP_240307068.1 NZ_CP092686:c46900-46496 [Vibrio campbellii]
MTAVDRICQKIKRSRRYVFERKDFDGFASYDQIGRALRQLVQQGELMKLGYGLYTKARINSLTGKPMPTNPGGTDALMRE
ILKMKGVDFEMDSLSLQSLSGTSTQIPSSIQYSWDPKQFNRKLVVGSRVLNLPS

Nucleotide

• Length: 405 bp

>AT236937 NZ_LR778141:c1184847-1184608 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA