Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 1456452..1457028 Replicon chromosome
Accession NZ_CP092681
Organism Leptospira interrogans strain FMAS_AP1

Toxin (Protein)


Gene name chpK Uniprot ID Q93MT8
Locus tag FH589_RS08000 Protein ID WP_000617906.1
Coordinates 1456687..1457028 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID Q93MT9
Locus tag FH589_RS07995 Protein ID WP_000844758.1
Coordinates 1456452..1456700 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH589_RS07980 (FH589_07990) 1452104..1454086 + 1983 WP_137170961.1 HD family phosphohydrolase -
FH589_RS07985 (FH589_07995) 1454727..1455185 - 459 WP_000453265.1 hypothetical protein -
FH589_RS07990 (FH589_08000) 1455339..1455635 + 297 WP_000477061.1 helix-turn-helix transcriptional regulator -
FH589_RS07995 (FH589_08005) 1456452..1456700 + 249 WP_000844758.1 ribbon-helix-helix domain-containing protein Antitoxin
FH589_RS08000 (FH589_08010) 1456687..1457028 + 342 WP_000617906.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FH589_RS08005 (FH589_08015) 1458053..1459045 - 993 WP_137108480.1 aldolase/citrate lyase family protein -
FH589_RS08010 (FH589_08020) 1459185..1460120 - 936 WP_000004530.1 ornithine carbamoyltransferase -
FH589_RS08015 (FH589_08025) 1460436..1460606 + 171 WP_154670643.1 hypothetical protein -
FH589_RS08020 (FH589_08030) 1460904..1461371 + 468 WP_000060053.1 hypothetical protein -
FH589_RS08025 (FH589_08035) 1461368..1461772 + 405 WP_001974506.1 pyridoxamine 5'-phosphate oxidase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12372.25 Da        Isoelectric Point: 6.3272

>T236935 WP_000617906.1 NZ_CP092681:1456687-1457028 [Leptospira interrogans]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGEVEIGLKLVTGLD

Download         Length: 342 bp

>T236935 NZ_LR778141:c806514-806227 [Escherichia coli]
ATGTTACCCATTTTATGGCTACCATCTGCTCGCGATGATTTGCGTCAGATCGTAGCCTATATTGCTAAGGAAAATATTCC
TGCAGCACGCAGACTAAAAATACGTATTGAAACGTCTGTTTTAGCCCTCTCCGAGCATCCATATCTATATCCGCCAAGTG
ATCGAGTATCCGGTTTGCGGGAAATTGTGGTTCACCCTAATTATATCGTTTTGTACCGAGTAGCAGCTTCAAGCATTGAA
ATTGCAAATATTGTGCATGCCCGCCGACAATTTCCCTTCCCTATCTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9380.77 Da        Isoelectric Point: 10.3815

>AT236935 WP_000844758.1 NZ_CP092681:1456452-1456700 [Leptospira interrogans]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT236935 NZ_LR778141:c806705-806514 [Escherichia coli]
ATGAGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCGC
TAAAGTGGCGTCAAGCCTTGCAGACCCTCGTCCCTCAATTCCACATGACGAGGTAATGGCTGAAATAGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAGGAGCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MS74

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HD92

References