Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 1035446..1036074 Replicon chromosome
Accession NZ_CP092681
Organism Leptospira interrogans strain FMAS_AP1

Toxin (Protein)


Gene name vapC Uniprot ID Q8F7E4
Locus tag FH589_RS06420 Protein ID WP_000281042.1
Coordinates 1035446..1035844 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8F7E3
Locus tag FH589_RS06425 Protein ID WP_001192482.1
Coordinates 1035844..1036074 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH589_RS06395 (FH589_06405) 1031178..1031945 + 768 WP_000636954.1 DUF1554 domain-containing protein -
FH589_RS06400 (FH589_06410) 1032464..1033324 + 861 WP_149792691.1 hypothetical protein -
FH589_RS06405 (FH589_06415) 1033580..1034656 - 1077 WP_001170569.1 hypothetical protein -
FH589_RS06410 (FH589_06420) 1034767..1035027 - 261 WP_239033688.1 hypothetical protein -
FH589_RS06415 (FH589_06425) 1035049..1035348 - 300 Protein_845 hypothetical protein -
FH589_RS06420 (FH589_06430) 1035446..1035844 - 399 WP_000281042.1 type II toxin-antitoxin system VapC family toxin Toxin
FH589_RS06425 (FH589_06435) 1035844..1036074 - 231 WP_001192482.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FH589_RS06430 (FH589_06440) 1036672..1037253 - 582 WP_137170912.1 hypothetical protein -
FH589_RS06435 (FH589_06445) 1037493..1038641 - 1149 WP_137170914.1 hypothetical protein -
FH589_RS06440 (FH589_06450) 1038826..1039941 - 1116 WP_000353261.1 protein kinase -
FH589_RS06445 (FH589_06455) 1039996..1040349 - 354 WP_149792689.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15139.76 Da        Isoelectric Point: 9.6893

>T236934 WP_000281042.1 NZ_CP092681:c1035844-1035446 [Leptospira interrogans]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Download         Length: 399 bp

>T236934 NZ_LR778141:c759035-758928 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8771.05 Da        Isoelectric Point: 8.6588

>AT236934 WP_001192482.1 NZ_CP092681:c1036074-1035844 [Leptospira interrogans]
MQTAKLFINGRSQAVRLPKEFQFTGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEEREQPKSDKREKL

Download         Length: 231 bp

>AT236934 NZ_LR778141:759082-759148 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6HAN6

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HAF7

References