Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF-MazE
Location 6878..7443 Replicon plasmid pLiSL24
Accession NZ_CP092680
Organism Leptospira interrogans strain FMAS_AP1

Toxin (Protein)


Gene name mazE Uniprot ID A0A0F6H5W7
Locus tag FH589_RS01890 Protein ID WP_000237067.1
Coordinates 6878..7201 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazF Uniprot ID A0A0F6H5X2
Locus tag FH589_RS01895 Protein ID WP_000648189.1
Coordinates 7195..7443 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH589_RS01860 (FH589_01865) 2863..3487 - 625 Protein_3 integrase core domain-containing protein -
FH589_RS01865 (FH589_01870) 3590..4786 + 1197 WP_000575407.1 kelch repeat-containing protein -
FH589_RS01870 (FH589_01875) 5180..5371 - 192 Protein_5 AraC family transcriptional regulator -
FH589_RS01875 (FH589_01880) 5664..6425 - 762 Protein_6 hypothetical protein -
FH589_RS01880 (FH589_01885) 6341..6520 + 180 WP_000536132.1 hypothetical protein -
FH589_RS01885 (FH589_01890) 6585..6770 - 186 Protein_8 toxin-antitoxin system, toxin component -
FH589_RS01890 (FH589_01895) 6878..7201 - 324 WP_000237067.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FH589_RS01895 (FH589_01900) 7195..7443 - 249 WP_000648189.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FH589_RS01900 (FH589_01905) 8385..8630 - 246 WP_000695324.1 hypothetical protein -
FH589_RS01905 (FH589_01910) 8750..9238 - 489 WP_002131542.1 DUF4942 domain-containing protein -
FH589_RS01910 (FH589_01915) 9370..9873 - 504 WP_000614121.1 hypothetical protein -
FH589_RS01915 (FH589_01920) 10120..10614 + 495 WP_017858174.1 hypothetical protein -
FH589_RS01920 (FH589_01925) 10863..11141 - 279 WP_000542660.1 hypothetical protein -
FH589_RS01925 (FH589_01930) 11387..11542 - 156 WP_002131775.1 hypothetical protein -
FH589_RS01930 (FH589_01935) 11692..11889 - 198 WP_239043567.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..64981 64981


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-104)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12096.52 Da        Isoelectric Point: 10.6275

>T236932 WP_000237067.1 NZ_CP092680:c7201-6878 [Leptospira interrogans]
MVISQYEVYLINLDPTVGHGIKKSRPCAIISPNEMNKTIGTIIIAPMTTKSRSYPTRVELTFQGKKGWIVLDQIRAVDKT
RLVKKLGKIDPKTVNKMKLVIKEMLVD

Download         Length: 324 bp

>T236932 NZ_LR778141:c757964-757857 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9507.04 Da        Isoelectric Point: 5.1749

>AT236932 WP_000648189.1 NZ_CP092680:c7443-7195 [Leptospira interrogans]
MKASVVKIGNSKGIRIPKAVLEECHIEEEVDLLIDNNKLIIVPLKSKPREGWEKQFKTMSSNKDDKLFIPDSIDLLDKDW
EW

Download         Length: 249 bp

>AT236932 NZ_LR778141:758012-758078 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6H5W7

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6H5X2

References