Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 75906..76477 Replicon plasmid pLiSL23
Accession NZ_CP092679
Organism Leptospira interrogans strain FMAS_AP1

Toxin (Protein)


Gene name mazF Uniprot ID Q8F5A3
Locus tag FH589_RS01810 Protein ID WP_000240418.1
Coordinates 75906..76247 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M6U553
Locus tag FH589_RS01815 Protein ID WP_000443825.1
Coordinates 76241..76477 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH589_RS01780 (FH589_01785) 71728..72339 - 612 WP_000851075.1 hypothetical protein -
FH589_RS01785 (FH589_01790) 73435..73635 - 201 WP_001206464.1 hypothetical protein -
FH589_RS01790 (FH589_01795) 74059..74784 + 726 WP_239033801.1 hypothetical protein -
FH589_RS01795 (FH589_01800) 74787..75128 + 342 WP_000727287.1 hypothetical protein -
FH589_RS01800 (FH589_01805) 75189..75455 + 267 WP_000844450.1 hypothetical protein -
FH589_RS01805 (FH589_01810) 75455..75838 + 384 WP_000617796.1 hypothetical protein -
FH589_RS01810 (FH589_01815) 75906..76247 - 342 WP_000240418.1 endoribonuclease MazF Toxin
FH589_RS01815 (FH589_01820) 76241..76477 - 237 WP_000443825.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FH589_RS01820 (FH589_01825) 76826..77617 - 792 WP_061243661.1 hypothetical protein -
FH589_RS01825 (FH589_01830) 78111..78356 + 246 WP_061226370.1 hypothetical protein -
FH589_RS01830 (FH589_01835) 78499..79638 + 1140 WP_239043563.1 leucine-rich repeat domain-containing protein -
FH589_RS01835 (FH589_01840) 79910..80848 + 939 WP_061234895.1 Rpn family recombination-promoting nuclease/putative transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..83121 83121


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-112)

Antitoxin

Antitoxin PemI-like

(1-78)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12918.12 Da        Isoelectric Point: 9.9595

>T236931 WP_000240418.1 NZ_CP092679:c76247-75906 [Leptospira interrogans]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Download         Length: 342 bp

>T236931 NZ_LR778140:c4820635-4820324 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8728.95 Da        Isoelectric Point: 5.6663

>AT236931 WP_000443825.1 NZ_CP092679:c76477-76241 [Leptospira interrogans]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Download         Length: 237 bp

>AT236931 NZ_LR778140:c4820323-4820033 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4D8SL30

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HC33

References