Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 3164569..3165197 Replicon chromosome
Accession NZ_CP092676
Organism Leptospira interrogans strain FMAS_AW3

Toxin (Protein)


Gene name vapC Uniprot ID Q8F7E4
Locus tag FH583_RS15705 Protein ID WP_000281042.1
Coordinates 3164799..3165197 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q72P17
Locus tag FH583_RS15700 Protein ID WP_001192484.1
Coordinates 3164569..3164799 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH583_RS15670 (FH583_15665) 3159711..3159920 - 210 WP_000041838.1 hypothetical protein -
FH583_RS15675 (FH583_15670) 3160052..3160681 + 630 WP_001081629.1 S24 family peptidase -
FH583_RS15680 (FH583_15675) 3160856..3161779 + 924 WP_000680712.1 M28 family peptidase -
FH583_RS15685 (FH583_15680) 3162040..3162727 + 688 Protein_2655 SH3 domain-containing protein -
FH583_RS15690 (FH583_15685) 3162957..3163634 + 678 WP_000071998.1 hypothetical protein -
FH583_RS15695 (FH583_15690) 3163857..3164201 + 345 WP_000392070.1 hypothetical protein -
FH583_RS15700 (FH583_15695) 3164569..3164799 + 231 WP_001192484.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FH583_RS15705 (FH583_15700) 3164799..3165197 + 399 WP_000281042.1 type II toxin-antitoxin system VapC family toxin Toxin
FH583_RS15710 (FH583_15705) 3165295..3165594 + 300 Protein_2660 hypothetical protein -
FH583_RS15715 (FH583_15710) 3165616..3165843 + 228 WP_000130601.1 hypothetical protein -
FH583_RS15720 (FH583_15715) 3165986..3167062 + 1077 WP_001170569.1 hypothetical protein -
FH583_RS15725 (FH583_15720) 3167063..3167521 + 459 WP_001239004.1 GNAT family N-acetyltransferase -
FH583_RS15730 (FH583_15725) 3167737..3168597 - 861 WP_000682235.1 hypothetical protein -
FH583_RS15735 (FH583_15730) 3169138..3169905 - 768 WP_000636952.1 DUF1554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15139.76 Da        Isoelectric Point: 9.6893

>T236930 WP_000281042.1 NZ_CP092676:3164799-3165197 [Leptospira interrogans]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Download         Length: 399 bp

>T236930 NZ_LR778140:c4543554-4543381 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8698.99 Da        Isoelectric Point: 9.8940

>AT236930 WP_001192484.1 NZ_CP092676:3164569-3164799 [Leptospira interrogans]
MQTAKLFINGRSQAVRLPKEFQFTGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEGREQPKSDKREKL

Download         Length: 231 bp

>AT236930 NZ_LR778140:c4543878-4543582 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6HAN6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2D7U1

References