Detailed information of TA system TA023693

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 3747346..3747874
• Replicon: chromosome
• Accession: NC_012560
• Organism: Azotobacter vinelandii DJ

Toxin (Protein)

• Gene name: relE
• Uniprot ID: M9YHR5
• Locus tag: AVIN_RS16840
• Protein ID: WP_012702214.1
• Coordinates: 3747593..3747874 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: M9YCW9
• Locus tag: AVIN_RS16835
• Protein ID: WP_012702213.1
• Coordinates: 3747346..3747606 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVIN_RS16810	3742894..3743442	-	549	WP_012702208.1	CDP-alcohol phosphatidyltransferase family protein	-
AVIN_RS16815	3743488..3744492	-	1005	WP_012702209.1	DUF2066 domain-containing protein	-
AVIN_RS16820	3744719..3745774	+	1056	WP_012702210.1	phosphoribosylformylglycinamidine cyclo-ligase	-
AVIN_RS16825	3745774..3746421	+	648	WP_012702211.1	phosphoribosylglycinamide formyltransferase	-
AVIN_RS16830	3746435..3747151	+	717	WP_012702212.1	DUF3108 domain-containing protein	-
AVIN_RS16835	3747346..3747606	+	261	WP_012702213.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
AVIN_RS16840	3747593..3747874	+	282	WP_012702214.1	type II toxin-antitoxin system YafQ family toxin	Toxin
AVIN_RS16845	3748200..3748739	-	540	WP_012702215.1	DUF2058 domain-containing protein	-
AVIN_RS16850	3749006..3749839	-	834	WP_012702216.1	nucleoside triphosphate pyrophosphohydrolase	-
AVIN_RS16855	3749927..3750265	-	339	WP_012702217.1	DUF3325 domain-containing protein	-
AVIN_RS16860	3750259..3751920	-	1662	WP_012702218.1	PepSY domain-containing protein	-
AVIN_RS16865	3751920..3752135	-	216	WP_012702219.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-71)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10724.16 Da
• Isoelectric Point: 7.0617

>T23693 WP_012702214.1 NC_012560:3747593-3747874 [Azotobacter vinelandii DJ]
VRTIKQTSQFKRDLQREAKGQHQATLEDDLLPVIEALAKDRPPDVLHRDHALTGNWVDHRDCHIKTDLVLLYRKPNDETL
QLVRLGSHSELGL

Nucleotide

• Length: 282 bp

>T23693 NC_012560:3747593-3747874 [Azotobacter vinelandii DJ]
GTGCGGACGATTAAGCAGACCAGCCAGTTCAAGCGCGACTTGCAGCGCGAAGCGAAGGGGCAGCACCAGGCGACGTTGGA
AGACGACCTGCTGCCGGTCATCGAGGCGCTGGCGAAGGATCGCCCTCCGGATGTCCTGCATCGTGACCATGCCCTGACGG
GCAACTGGGTGGATCATCGCGACTGCCACATAAAAACGGACCTGGTGTTGCTGTACCGGAAGCCGAACGACGAAACGCTG
CAATTGGTTCGCCTCGGCTCGCATAGCGAGCTGGGGCTCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9395.79 Da
• Isoelectric Point: 4.4012

>AT23693 WP_012702213.1 NC_012560:3747346-3747606 [Azotobacter vinelandii DJ]
MATETTVRARIDERIKNEATVVLESMGLTMSDAIKLMLVRVAEEGRLPFAPLEPSLETIAAARAAREGNLETVTLDELRA
AIRADD

Nucleotide

• Length: 261 bp

>AT23693 NC_012560:3747346-3747606 [Azotobacter vinelandii DJ]
ATGGCCACCGAAACGACCGTCCGCGCCCGCATCGACGAGCGCATCAAGAATGAAGCCACCGTGGTGCTCGAATCCATGGG
CCTGACCATGTCGGACGCCATCAAGCTGATGCTGGTGCGCGTCGCCGAGGAAGGGCGCTTGCCGTTCGCGCCGCTGGAGC
CCTCCCTGGAAACCATCGCGGCTGCACGTGCAGCACGCGAGGGGAACCTCGAAACCGTGACCCTGGACGAGCTGCGGGCG
GCGATTCGTGCGGACGATTAA

Structures

Toxin

• AlphaFold DB: M9YHR5

Antitoxin

• AlphaFold DB: M9YCW9