Detailed information of TA system TA236929

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpIK/PemK(toxin)
• Location: 2746739..2747315
• Replicon: chromosome
• Accession: NZ_CP092676
• Organism: Leptospira interrogans strain FMAS_AW3

Toxin (Protein)

• Gene name: chpK
• Uniprot ID: -
• Locus tag: FH583_RS14105
• Protein ID: WP_000617908.1
• Coordinates: 2746739..2747080 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: chpI
• Uniprot ID: Q93MT9
• Locus tag: FH583_RS14110
• Protein ID: WP_000844758.1
• Coordinates: 2747067..2747315 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH583_RS14085 (FH583_14080)	2741972..2742376	-	405	WP_001974506.1	pyridoxamine 5'-phosphate oxidase family protein	-
FH583_RS14090 (FH583_14085)	2742373..2742840	-	468	WP_000060053.1	hypothetical protein	-
FH583_RS14095 (FH583_14090)	2743644..2744579	+	936	WP_000004530.1	ornithine carbamoyltransferase	-
FH583_RS14100 (FH583_14095)	2744719..2745711	+	993	WP_000041093.1	aldolase/citrate lyase family protein	-
FH583_RS14105 (FH583_14100)	2746739..2747080	-	342	WP_000617908.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FH583_RS14110 (FH583_14105)	2747067..2747315	-	249	WP_000844758.1	ribbon-helix-helix domain-containing protein	Antitoxin
FH583_RS14115 (FH583_14110)	2748130..2748426	-	297	WP_000477061.1	helix-turn-helix transcriptional regulator	-
FH583_RS14120 (FH583_14115)	2748580..2749038	+	459	WP_000453267.1	hypothetical protein	-
FH583_RS14125 (FH583_14120)	2749680..2751662	-	1983	WP_000802799.1	HD family phosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (2-41)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12371.30 Da
• Isoelectric Point: 9.7995

>T236929 WP_000617908.1 NZ_CP092676:c2747080-2746739 [Leptospira interrogans]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGKVEIGLKLVTGLD

Nucleotide

• Length: 342 bp

>T236929 NZ_LR778140:4530109-4530486 [Escherichia coli]
ATGAATACATTACCTGTATTACCCGGACAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGTCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCTCGGGTAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9380.77 Da
• Isoelectric Point: 10.3815

>AT236929 WP_000844758.1 NZ_CP092676:c2747315-2747067 [Leptospira interrogans]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Nucleotide

• Length: 249 bp

>AT236929 NZ_LR778140:4529651-4530019 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGACGACAACAACGAGCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCTTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0F6HD92