236926

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazE-MazF
Location 21970..22535 Replicon plasmid pLiSL29
Accession NZ_CP092670
Organism Leptospira interrogans strain FMAS_KG1

Toxin (Protein)


Gene name mazF Uniprot ID A0A0F6H5X2
Locus tag FH587_RS03450 Protein ID WP_000648189.1
Coordinates 21970..22218 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A0C5XAD8
Locus tag FH587_RS03455 Protein ID WP_000237066.1
Coordinates 22212..22535 (+) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH587_RS03430 (FH587_03430) 17740..18357 - 618 WP_061284347.1 hypothetical protein -
FH587_RS03435 (FH587_03435) 19317..19793 + 477 WP_240303685.1 hypothetical protein -
FH587_RS03440 (FH587_03440) 20065..20298 - 234 WP_240303686.1 hypothetical protein -
FH587_RS03445 (FH587_03445) 20329..20706 - 378 WP_017853608.1 hypothetical protein -
FH587_RS03450 (FH587_03450) 21970..22218 + 249 WP_000648189.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Toxin
FH587_RS03455 (FH587_03455) 22212..22535 + 324 WP_000237066.1 type II toxin-antitoxin system PemK/MazF family toxin Antitoxin
FH587_RS03460 (FH587_03460) 22643..22828 + 186 Protein_33 toxin-antitoxin system, toxin component -
FH587_RS03465 (FH587_03465) 22892..23071 - 180 WP_000536132.1 hypothetical protein -
FH587_RS03470 (FH587_03470) 22987..23748 + 762 Protein_35 hypothetical protein -
FH587_RS03475 (FH587_03475) 24041..24232 + 192 Protein_36 AraC family transcriptional regulator -
FH587_RS03480 (FH587_03480) 24617..25495 - 879 Protein_37 kelch repeat-containing protein -
FH587_RS03485 (FH587_03485) 25745..26818 + 1074 WP_162303305.1 IS110-like element ISLin1 family transposase -
FH587_RS23160 26857..27175 - 319 Protein_39 galactose oxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..96233 96233
- flank IS/Tn - - 25745..26818 1073


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

SpoVT-AbrB domain

(6-49)

Antitoxin

mRNA interferase PemK-like

(2-105)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9507.04 Da        Isoelectric Point: 5.1749

>T236926 WP_000648189.1 NZ_CP092670:21970-22218 [Leptospira interrogans]
MKASVVKIGNSKGIRIPKAVLEECHIEEEVDLLIDNNKLIIVPLKSKPREGWEKQFKTMSSNKDDKLFIPDSIDLLDKDW
EW

Download         Length: 249 bp

>T236926 NZ_LR778140:3678282-3678824 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12168.59 Da        Isoelectric Point: 10.5161

>AT236926 WP_000237066.1 NZ_CP092670:22212-22535 [Leptospira interrogans]
MVISQYEVYLINLDPTVGHEIKKSRPCAIISPNEMNKTIGTIIIAPMTTKSRSYPTRVELTFQGKKGWIVLDQIRAVDKT
RLVKKLGKIDPKTVNKMKLVIKEMLVD

Download         Length: 324 bp

>AT236926 NZ_LR778140:3677987-3678298 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6H5X2

Antitoxin

Source ID Structure
AlphaFold DB A0A0C5XAD8

References