Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 75881..76452 Replicon plasmid pLiSL28
Accession NZ_CP092669
Organism Leptospira interrogans strain FMAS_KG1

Toxin (Protein)


Gene name mazF Uniprot ID Q8F5A3
Locus tag FH587_RS03220 Protein ID WP_000240418.1
Coordinates 75881..76222 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M6U553
Locus tag FH587_RS03225 Protein ID WP_000443825.1
Coordinates 76216..76452 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH587_RS03210 (FH587_03210) 71110..72123 - 1014 WP_000427241.1 hypothetical protein -
FH587_RS03215 (FH587_03215) 74320..75731 + 1412 Protein_62 transposase -
FH587_RS03220 (FH587_03220) 75881..76222 - 342 WP_000240418.1 endoribonuclease MazF Toxin
FH587_RS03225 (FH587_03225) 76216..76452 - 237 WP_000443825.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FH587_RS03230 (FH587_03230) 76789..77211 - 423 WP_000402980.1 hypothetical protein -
FH587_RS03235 (FH587_03235) 77260..77472 - 213 WP_000447737.1 hypothetical protein -
FH587_RS03240 (FH587_03240) 78644..79942 + 1299 WP_000558495.1 group II intron reverse transcriptase/maturase -
FH587_RS03245 (FH587_03245) 80300..81148 - 849 WP_082270396.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..88750 88750


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-112)

Antitoxin

Antitoxin PemI-like

(1-78)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12918.12 Da        Isoelectric Point: 9.9595

>T236925 WP_000240418.1 NZ_CP092669:c76222-75881 [Leptospira interrogans]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Download         Length: 342 bp

>T236925 NZ_LR778140:3644311-3644529 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8728.95 Da        Isoelectric Point: 5.6663

>AT236925 WP_000443825.1 NZ_CP092669:c76452-76216 [Leptospira interrogans]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Download         Length: 237 bp

>AT236925 NZ_LR778140:3643911-3644285 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4D8SL30

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HC33

References