236924

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 1442613..1443189 Replicon chromosome
Accession NZ_CP092663
Organism Leptospira interrogans strain FMAS_PN3

Toxin (Protein)


Gene name chpK Uniprot ID Q93MT8
Locus tag FH598_RS06260 Protein ID WP_000617906.1
Coordinates 1442848..1443189 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID Q93MT9
Locus tag FH598_RS06255 Protein ID WP_000844758.1
Coordinates 1442613..1442861 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH598_RS06240 (FH598_06225) 1438265..1440247 + 1983 WP_137170961.1 HD family phosphohydrolase -
FH598_RS06245 (FH598_06230) 1440888..1441346 - 459 WP_000453265.1 hypothetical protein -
FH598_RS06250 (FH598_06235) 1441500..1441796 + 297 WP_000477061.1 helix-turn-helix transcriptional regulator -
FH598_RS06255 (FH598_06240) 1442613..1442861 + 249 WP_000844758.1 ribbon-helix-helix domain-containing protein Antitoxin
FH598_RS06260 (FH598_06245) 1442848..1443189 + 342 WP_000617906.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FH598_RS06265 (FH598_06250) 1444214..1445206 - 993 WP_137108480.1 aldolase/citrate lyase family protein -
FH598_RS06270 (FH598_06255) 1445346..1446281 - 936 WP_000004530.1 ornithine carbamoyltransferase -
FH598_RS06275 1446597..1446767 + 171 WP_154670643.1 hypothetical protein -
FH598_RS06280 (FH598_06260) 1447065..1447532 + 468 WP_000060053.1 hypothetical protein -
FH598_RS06285 (FH598_06265) 1447529..1447933 + 405 WP_001974506.1 pyridoxamine 5'-phosphate oxidase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12372.25 Da        Isoelectric Point: 6.3272

>T236924 WP_000617906.1 NZ_CP092663:1442848-1443189 [Leptospira interrogans]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGEVEIGLKLVTGLD

Download         Length: 342 bp

>T236924 NZ_LR778140:2790898-2791005 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9380.77 Da        Isoelectric Point: 10.3815

>AT236924 WP_000844758.1 NZ_CP092663:1442613-1442861 [Leptospira interrogans]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT236924 NZ_LR778140:c2790848-2790785 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MS74

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HD92

References