Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 86114..86685 Replicon plasmid pLiSL33
Accession NZ_CP092662
Organism Leptospira interrogans strain FMAS_PN3

Toxin (Protein)


Gene name mazF Uniprot ID Q8F5A3
Locus tag FH598_RS00320 Protein ID WP_000240418.1
Coordinates 86344..86685 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M6U553
Locus tag FH598_RS00315 Protein ID WP_000443825.1
Coordinates 86114..86350 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH598_RS00295 (FH598_00295) 81743..82681 - 939 WP_061234895.1 Rpn family recombination-promoting nuclease/putative transposase -
FH598_RS00300 (FH598_00300) 82953..84092 - 1140 WP_239043563.1 leucine-rich repeat domain-containing protein -
FH598_RS00305 (FH598_00305) 84235..84480 - 246 WP_061226370.1 hypothetical protein -
FH598_RS00310 (FH598_00310) 84974..85765 + 792 WP_061243661.1 hypothetical protein -
FH598_RS00315 (FH598_00315) 86114..86350 + 237 WP_000443825.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FH598_RS00320 (FH598_00320) 86344..86685 + 342 WP_000240418.1 endoribonuclease MazF Toxin
FH598_RS00325 (FH598_00325) 86753..87136 - 384 WP_000617796.1 hypothetical protein -
FH598_RS00330 (FH598_00330) 87136..87402 - 267 WP_000844450.1 hypothetical protein -
FH598_RS00335 (FH598_00335) 87463..87804 - 342 WP_000727287.1 hypothetical protein -
FH598_RS00340 (FH598_00340) 87807..88532 - 726 WP_239033801.1 hypothetical protein -
FH598_RS00345 (FH598_00345) 88956..89156 + 201 WP_001206464.1 hypothetical protein -
FH598_RS00350 (FH598_00350) 90252..90863 + 612 WP_000851075.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..100013 100013


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-112)

Antitoxin

Antitoxin PemI-like

(1-78)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12918.12 Da        Isoelectric Point: 9.9595

>T236922 WP_000240418.1 NZ_CP092662:86344-86685 [Leptospira interrogans]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Download         Length: 342 bp

>T236922 NZ_LR778140:2789827-2789934 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8728.95 Da        Isoelectric Point: 5.6663

>AT236922 WP_000443825.1 NZ_CP092662:86114-86350 [Leptospira interrogans]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Download         Length: 237 bp

>AT236922 NZ_LR778140:c2789780-2789714 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4D8SL30

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HC33

References