Detailed information of TA system TA023692

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 3703859..3704388
• Replicon: chromosome
• Accession: NC_012560
• Organism: Azotobacter vinelandii DJ

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: M9YLU7
• Locus tag: AVIN_RS16580
• Protein ID: WP_012702163.1
• Coordinates: 3703859..3704182 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AVIN_RS16585
• Protein ID: WP_041807346.1
• Coordinates: 3704179..3704388 (-)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVIN_RS16555	3699059..3699991	+	933	WP_012702159.1	TIGR03756 family integrating conjugative element protein	-
AVIN_RS16560	3700014..3701378	+	1365	WP_012702160.1	integrating conjugative element protein	-
AVIN_RS16565	3701385..3701741	+	357	WP_012702161.1	hypothetical protein	-
AVIN_RS16570	3701738..3703303	+	1566	WP_012702162.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
AVIN_RS16575	3703321..3703626	-	306	WP_041807344.1	hypothetical protein	-
AVIN_RS25370	3703679..3703852	-	174	WP_081434905.1	hypothetical protein	-
AVIN_RS16580	3703859..3704182	-	324	WP_012702163.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AVIN_RS16585	3704179..3704388	-	210	WP_041807346.1	antitoxin MazE family protein	Antitoxin
AVIN_RS16590	3704582..3705184	+	603	WP_012702164.1	DUF4852 domain-containing protein	-
AVIN_RS16595	3705297..3705536	-	240	WP_175556057.1	Arc family DNA-binding protein	-
AVIN_RS16600	3705886..3706919	+	1034	Protein_3320	IS630 family transposase	-
AVIN_RS25380	3707336..3707974	+	639	WP_012702167.1	hypothetical protein	-
AVIN_RS16605	3708196..3708903	-	708	WP_012702168.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3617324..3712022	94698

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (5-68)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11433.43 Da
• Isoelectric Point: 9.7779

>T23692 WP_012702163.1 NC_012560:c3704182-3703859 [Azotobacter vinelandii DJ]
MNRGAVVVVALQGAYGKPRPALVIQSDLFSALPSVTILPITSDLREAPLYRIDIEAAPRTGLHKPSQIMVDKPQTIPREK
VGEVIGSVNAETMLAVSRSLTVFLGIA

Nucleotide

• Length: 324 bp

>T23692 NC_012560:c3704182-3703859 [Azotobacter vinelandii DJ]
ATGAATCGAGGTGCCGTGGTGGTGGTCGCCTTGCAGGGCGCCTACGGAAAGCCTCGCCCGGCGTTGGTCATCCAGTCCGA
TCTGTTCTCGGCGTTGCCTTCCGTGACCATTCTTCCTATCACGAGCGATTTGCGGGAGGCGCCGCTTTATCGCATCGACA
TCGAGGCCGCGCCGAGAACCGGGTTGCACAAGCCATCACAGATCATGGTGGACAAGCCGCAAACGATCCCCCGCGAGAAA
GTGGGCGAGGTGATCGGCTCGGTCAACGCAGAAACCATGCTGGCCGTGAGTCGGTCGCTAACCGTGTTTCTCGGTATTGC
TTGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8061.07 Da
• Isoelectric Point: 5.1502

>AT23692 WP_041807346.1 NC_012560:c3704388-3704179 [Azotobacter vinelandii DJ]
MQTVRERVRKRREALRAAGLRPVQIWVPDTRAPGFAEEARRQCLLVQNDPQEKESLEWLESAADTEGWE

Nucleotide

• Length: 210 bp

>AT23692 NC_012560:c3704388-3704179 [Azotobacter vinelandii DJ]
ATGCAGACCGTGCGTGAGCGGGTCAGGAAGCGGCGCGAGGCGCTGCGGGCTGCTGGGTTGCGCCCGGTGCAAATCTGGGT
GCCGGACACCCGTGCTCCCGGCTTTGCGGAAGAGGCGAGGCGGCAGTGCCTTCTCGTACAGAATGATCCGCAAGAGAAAG
AAAGTCTGGAGTGGCTGGAAAGCGCGGCTGACACAGAGGGGTGGGAATGA

Structures

Toxin

• AlphaFold DB: M9YLU7