Detailed information of TA system TA236902

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 273341..273863
• Replicon: plasmid pNB5_NDM
• Accession: NZ_CP092653
• Organism: Klebsiella pneumoniae strain NB5

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A7H0EVH6
• Locus tag: MJ642_RS01510
• Protein ID: WP_014839901.1
• Coordinates: 273579..273863 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A7H0EVH5
• Locus tag: MJ642_RS01505
• Protein ID: WP_014839900.1
• Coordinates: 273341..273589 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ642_RS01480	268519..268875	+	357	WP_238050194.1	hypothetical protein	-
MJ642_RS01485	269249..269647	+	399	WP_014839899.1	H-NS family nucleoid-associated regulatory protein	-
MJ642_RS01490	269784..270392	+	609	WP_024266306.1	DUF2913 family protein	-
MJ642_RS01495	270936..271568	+	633	WP_001567369.1	hypothetical protein	-
MJ642_RS01500	271597..273000	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
MJ642_RS01505	273341..273589	+	249	WP_014839900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MJ642_RS01510	273579..273863	+	285	WP_014839901.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MJ642_RS01515	274054..274530	+	477	WP_042934526.1	hypothetical protein	-
MJ642_RS01520	274536..275213	+	678	WP_042934501.1	hypothetical protein	-
MJ642_RS01525	275230..275571	-	342	WP_233430673.1	DUF2807 domain-containing protein	-
MJ642_RS01530	275554..275949	-	396	Protein_306	cation:proton antiporter	-
MJ642_RS01535	276190..276792	-	603	WP_014839902.1	HAD domain-containing protein	-
MJ642_RS01540	276789..277310	-	522	WP_048336588.1	hypothetical protein	-
MJ642_RS01545	277391..278335	-	945	WP_014839903.1	DsbA family protein	-
MJ642_RS01550	278398..278607	-	210	WP_125316032.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(E) / msr(E) / armA / sul1 / qacE / aadA5 / dfrA1 / blaNDM-1 / fosA3 / blaSHV-12 / qnrB4 / blaDHA-1 / qnrB52 / mph(A)	htpB	1..355489	355489
	-	flank	IS/Tn	-	-	271597..273000	1403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10866.46 Da
• Isoelectric Point: 10.5831

>T236902 WP_014839901.1 NZ_CP092653:273579-273863 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLSERLQNPRVPAAQLHGGKDQYKIKLHGAGYRLVYSVNDEIVTVTVIGVGK
RNNDDIYNATRHRN

Nucleotide

• Length: 285 bp

>T236902 NZ_LR745045:c5245119-5244736 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACTTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9071.29 Da
• Isoelectric Point: 3.8832

>AT236902 WP_014839900.1 NZ_CP092653:273341-273589 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMEMLEDQELGRIADERIGEDRIEVNID
DL

Nucleotide

• Length: 249 bp

>AT236902 NZ_LR745045:c5245361-5245119 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A7H0EVH6

Antitoxin

• AlphaFold DB: A0A7H0EVH5