Detailed information of TA system TA236895

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1161346..1162969
• Replicon: chromosome
• Accession: NZ_CP092652
• Organism: Proteus mirabilis strain PM8762

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: H9TH67
• Locus tag: MJ616_RS05535
• Protein ID: WP_000088046.1
• Coordinates: 1161346..1162671 (-)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: MJ616_RS05540
• Protein ID: WP_024136327.1
• Coordinates: 1162676..1162969 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ616_RS05500	1157065..1157268	-	204	WP_001194555.1	hypothetical protein	-
MJ616_RS05505	1157330..1158187	-	858	WP_000784387.1	HNH endonuclease signature motif containing protein	-
MJ616_RS05510	1158203..1158511	-	309	WP_001224687.1	hypothetical protein	-
MJ616_RS05515	1159060..1159485	-	426	WP_001043843.1	hypothetical protein	-
MJ616_RS05520	1159739..1160554	-	816	WP_001572439.1	HNH endonuclease signature motif containing protein	-
MJ616_RS05525	1160567..1160977	-	411	WP_000985911.1	hypothetical protein	-
MJ616_RS05530	1161079..1161285	-	207	WP_000134171.1	hypothetical protein	-
MJ616_RS05535	1161346..1162671	-	1326	WP_000088046.1	type II toxin-antitoxin system HipA family toxin	Toxin
MJ616_RS05540	1162676..1162969	-	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
MJ616_RS05545	1163573..1163965	-	393	WP_000341066.1	hypothetical protein	-
MJ616_RS05550	1164338..1164679	+	342	WP_001151575.1	hypothetical protein	-
MJ616_RS05555	1164771..1164962	+	192	WP_000398480.1	hypothetical protein	-
MJ616_RS05560	1165012..1165293	+	282	WP_000077926.1	hypothetical protein	-
MJ616_RS19920	1165634..1165765	-	132	WP_000374059.1	hypothetical protein	-
MJ616_RS05565	1165856..1167097	-	1242	WP_001053338.1	VWA domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	blaNDM-5 / mph(A) / sul1 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / blaOXA-1 / catB3	-	1094104..1389516	295412

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (9-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50249.20 Da
• Isoelectric Point: 6.1927

>T236895 WP_000088046.1 NZ_CP092652:c1162671-1161346 [Proteus mirabilis]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T236895 NZ_LR745045:1982259-1982361 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT236895 WP_024136327.1 NZ_CP092652:c1162969-1162676 [Proteus mirabilis]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

>AT236895 NZ_LR745045:c1982368-1982223 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Toxin

• AlphaFold DB: A0A3T0VBU1

Antitoxin

• AlphaFold DB: A0A5Z6V2D1