Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5108671..5109296 Replicon chromosome
Accession NZ_CP092648
Organism Klebsiella pneumoniae strain KP7728

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MJ615_RS24840 Protein ID WP_002882817.1
Coordinates 5108671..5109054 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MJ615_RS24845 Protein ID WP_004150355.1
Coordinates 5109054..5109296 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ615_RS24825 (5106037) 5106037..5106939 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MJ615_RS24830 (5106936) 5106936..5107571 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MJ615_RS24835 (5107568) 5107568..5108497 + 930 WP_240289430.1 formate dehydrogenase accessory protein FdhE -
MJ615_RS24840 (5108671) 5108671..5109054 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MJ615_RS24845 (5109054) 5109054..5109296 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MJ615_RS24850 (5109501) 5109501..5110418 + 918 WP_023302328.1 alpha/beta hydrolase -
MJ615_RS24855 (5110432) 5110432..5111373 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MJ615_RS24860 (5111418) 5111418..5111855 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MJ615_RS24865 (5111852) 5111852..5112712 - 861 WP_004146232.1 virulence factor BrkB family protein -
MJ615_RS24870 (5112706) 5112706..5113305 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T236892 WP_002882817.1 NZ_CP092648:c5109054-5108671 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T236892 NZ_LR745045:323624-323992 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT236892 WP_004150355.1 NZ_CP092648:c5109296-5109054 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT236892 NZ_LR745045:323406-323627 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References