Detailed information of TA system TA236873

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4839745..4840383
• Replicon: chromosome
• Accession: NZ_CP092647
• Organism: Escherichia coli strain EC5502

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: MJ614_RS25640
• Protein ID: WP_000813794.1
• Coordinates: 4839745..4839921 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: MJ614_RS25645
• Protein ID: WP_001270285.1
• Coordinates: 4839967..4840383 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ614_RS25620 (4835364)	4835364..4836539	-	1176	WP_001236265.1	BenE family transporter YdcO	-
MJ614_RS25625 (4836631)	4836631..4837167	+	537	WP_000429153.1	DNA-binding transcriptional regulator SutR	-
MJ614_RS25630 (4837240)	4837240..4839201	+	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
MJ614_RS25635 (4839293)	4839293..4839523	-	231	WP_000494244.1	YncJ family protein	-
MJ614_RS25640 (4839745)	4839745..4839921	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
MJ614_RS25645 (4839967)	4839967..4840383	+	417	WP_001270285.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
MJ614_RS25650 (4840462)	4840462..4841868	+	1407	WP_000760590.1	PLP-dependent aminotransferase family protein	-
MJ614_RS25655 (4842113)	4842113..4843258	+	1146	WP_000034363.1	ABC transporter substrate-binding protein	-
MJ614_RS25660 (4843276)	4843276..4844289	+	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
MJ614_RS25665 (4844290)	4844290..4845231	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4834176..4835324	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T236873 WP_000813794.1 NZ_CP092647:4839745-4839921 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T236873 NZ_LR740777:71738-72136 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATGAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAGCTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15247.61 Da
• Isoelectric Point: 4.6115

>AT236873 WP_001270285.1 NZ_CP092647:4839967-4840383 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT236873 NZ_LR740777:71511-71738 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• PDB: 6HPB