Detailed information of TA system TA236867

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3740117..3740954
• Replicon: chromosome
• Accession: NZ_CP092647
• Organism: Escherichia coli strain EC5502

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: MJ614_RS20210
• Protein ID: WP_240296997.1
• Coordinates: 3740117..3740659 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: MJ614_RS20215
• Protein ID: WP_001297137.1
• Coordinates: 3740643..3740954 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ614_RS20190 (3735656)	3735656..3736567	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
MJ614_RS20195 (3736735)	3736735..3737226	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
MJ614_RS20200 (3737354)	3737354..3738718	-	1365	WP_001000978.1	MFS transporter	-
MJ614_RS20205 (3739126)	3739126..3740061	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
MJ614_RS20210 (3740117)	3740117..3740659	-	543	WP_240296997.1	GNAT family N-acetyltransferase	Toxin
MJ614_RS20215 (3740643)	3740643..3740954	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
MJ614_RS20220 (3741139)	3741139..3742029	-	891	WP_000971332.1	heme o synthase	-
MJ614_RS20225 (3742041)	3742041..3742370	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
MJ614_RS20230 (3742370)	3742370..3742984	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
MJ614_RS20235 (3742974)	3742974..3744965	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
MJ614_RS20240 (3744987)	3744987..3745934	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (67-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19832.04 Da
• Isoelectric Point: 8.3395

>T236867 WP_240296997.1 NZ_CP092647:c3740659-3740117 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNNDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T236867 NZ_LR740776:4307033-4307344 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT236867 WP_001297137.1 NZ_CP092647:c3740954-3740643 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT236867 NZ_LR740776:4307345-4307635 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3