Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2184238..2184869 Replicon chromosome
Accession NZ_CP092647
Organism Escherichia coli strain EC5502

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag MJ614_RS12940 Protein ID WP_001259388.1
Coordinates 2184594..2184869 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag MJ614_RS12935 Protein ID WP_000593555.1
Coordinates 2184238..2184597 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ614_RS12910 (2180370) 2180370..2181314 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
MJ614_RS12915 (2181311) 2181311..2182144 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
MJ614_RS12920 (2182144) 2182144..2182908 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
MJ614_RS12925 (2182905) 2182905..2183711 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
MJ614_RS12930 (2183717) 2183717..2184118 + 402 WP_001190064.1 nickel-responsive transcriptional regulator NikR -
MJ614_RS12935 (2184238) 2184238..2184597 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MJ614_RS12940 (2184594) 2184594..2184869 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
MJ614_RS12945 (2184942) 2184942..2186066 - 1125 WP_001615305.1 ABC transporter permease -
MJ614_RS12950 (2186066) 2186066..2188801 - 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
MJ614_RS12955 (2188798) 2188798..2189865 - 1068 WP_000361482.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T236861 WP_001259388.1 NZ_CP092647:c2184869-2184594 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T236861 NZ_LR740776:2106504-2106878 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT236861 WP_000593555.1 NZ_CP092647:c2184597-2184238 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT236861 NZ_LR740776:2106047-2106415 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References