Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1672494..1673292 Replicon chromosome
Accession NZ_CP092647
Organism Escherichia coli strain EC5502

Toxin (Protein)


Gene name yeeV Uniprot ID U9XMP3
Locus tag MJ614_RS10360 Protein ID WP_000854735.1
Coordinates 1672915..1673292 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A1M1EW42
Locus tag MJ614_RS10355 Protein ID WP_032153712.1
Coordinates 1672494..1672868 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ614_RS10320 (1668227) 1668227..1669111 + 885 WP_024166630.1 50S ribosome-binding GTPase -
MJ614_RS10325 (1669230) 1669230..1669907 + 678 WP_023155728.1 hypothetical protein -
MJ614_RS10330 (1669937) 1669937..1670146 + 210 WP_023155727.1 DUF905 family protein -
MJ614_RS10335 (1670237) 1670237..1671055 + 819 WP_001234693.1 DUF932 domain-containing protein -
MJ614_RS10340 (1671147) 1671147..1671632 + 486 WP_032153711.1 antirestriction protein -
MJ614_RS10345 (1671648) 1671648..1672124 + 477 WP_001186715.1 RadC family protein -
MJ614_RS10350 (1672193) 1672193..1672414 + 222 WP_000692345.1 DUF987 domain-containing protein -
MJ614_RS10355 (1672494) 1672494..1672868 + 375 WP_032153712.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
MJ614_RS10360 (1672915) 1672915..1673292 + 378 WP_000854735.1 TA system toxin CbtA family protein Toxin
MJ614_RS10365 (1673289) 1673289..1673780 + 492 WP_023155722.1 DUF5983 family protein -
MJ614_RS10370 (1673792) 1673792..1673989 + 198 WP_000839265.1 DUF957 domain-containing protein -
MJ614_RS10375 (1674074) 1674074..1674940 + 867 WP_032153714.1 DUF4942 domain-containing protein -
MJ614_RS10380 (1675012) 1675012..1675275 + 264 WP_025492088.1 type II toxin-antitoxin system ParD family antitoxin -
MJ614_RS10385 (1675272) 1675272..1675598 + 327 WP_000779483.1 type II toxin-antitoxin system RelE/ParE family toxin -
MJ614_RS10390 (1676013) 1676013..1676549 - 537 WP_000942786.1 GspM family type II secretion system protein YghD -
MJ614_RS10395 (1676551) 1676551..1677729 - 1179 WP_000094974.1 type II secretion system protein GspL -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 papB / papI 1608117..1675598 67481


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14061.07 Da        Isoelectric Point: 8.2904

>T236859 WP_000854735.1 NZ_CP092647:1672915-1673292 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T236859 NZ_LR740776:c1588692-1587370 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGAGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAATGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCAAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACAGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTATGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGGTCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13697.51 Da        Isoelectric Point: 6.6240

>AT236859 WP_032153712.1 NZ_CP092647:1672494-1672868 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT236859 NZ_LR740776:c1588958-1588692 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAATGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTAACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCAAAAAATGCCTCGCCAGAAGCA
ACAGAACAGCAAGATCTGGAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XMP3

Antitoxin

Source ID Structure
AlphaFold DB A0A1M1EW42

References